Supramolecular hemoprotein linear assembly by successive interprotein heme-heme pocket interactions
Supramolecular hemoprotein linear assembly by successive interprotein heme-heme pocket interactions
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DOI：
10.1021/ja073295q
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发表时间：
2007-08-29
影响因子：
15
通讯作者：
Hayashi, Takashi
中科院分区：
文献类型：
--
作者：
Kitagishi, Hiroaki;Oohora, Koji;Hayashi, Takashi
我们展示了一种构建超分子血红蛋白组装体的新策略。将合成血红素通过硫醚键选择性地引入细胞色素b（562）单突变体（H63C）的Cys残基表面。通过酸变性和中和去除H63C突变体的天然血红素后，载脂蛋白表面的外部附着血红素被插入到另一个载脂蛋白的空血红素口袋中。因此，蛋白间血红素-血红素口袋相互作用产生独特的亚微米大小的线性血红蛋白纤维，通过尺寸排除色谱和原子力显微镜确定。这种方法应该广泛适用于基于功能性血红蛋白的新型纳米生物材料的创造。
We demonstrate a new strategy for the construction of supramolecular hemoprotein assemblies. A synthetic heme was selectively introduced onto the surface Cys residue of the cytochrome b(562) single mutant (H63C) through a thioether bond. After removal of the native heme of the H63C mutant by acid denaturation followed by neutralization, the externally attached heme on the apoprotein surface was inserted into the vacant heme pocket of the other apoprotein. Therefore, the interprotein heme-heme pocket interaction produces a unique submicrometer-sized linear hemoprotein fiber, determined by size exclusion chromatography and atomic force microscopy. This methodology should be widely applicable to the creation of new nanobiomaterials based on a functional hemoprotein.