Viral suppressors of RNA silencing hinder exogenous and endogenous small RNA pathways in Drosophila.
Viral suppressors of RNA silencing hinder exogenous and endogenous small RNA pathways in Drosophila.
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DOI：
10.1371/journal.pone.0005866
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发表时间：
2009-06-10
期刊：
影响因子：
3.7
通讯作者：
Antoniewski C
中科院分区：
文献类型：
--
作者：
Berry B;Deddouche S;Kirschner D;Imler JL;Antoniewski C
在植物和昆虫中，RNA干扰（RNAi）是对抗病毒的主要应答者，并形成抗病毒免疫的基础。病毒通过表达RNAi的病毒抑制子（VSRs）来对抗这种防御。虽然黑腹果蝇中的VSR显示出通过不同的作用模式抑制RNAi，但它们是否作用于其他沉默途径仍未探索。在这里，我们表明转基因苍蝇中各种植物和昆虫VSR的表达不会扰乱果蝇微小RNA（miRNA）途径;但相反，抑制抗病毒RNA干扰和反向重复转录物以及注射双链RNA或小干扰RNA触发的RNA沉默反应。引人注目的是，这些VSR还抑制了内源性siRNA（endo-siRNA）引起的转座子沉默。我们的研究结果确定了VSR作为解开昆虫中小RNA通路的工具，并表明在苍蝇感染期间病毒对抗病毒RNAi和endo-siRNA沉默的共同抑制。
In plants and insects, RNA interference (RNAi) is the main responder against viruses and shapes the basis of antiviral immunity. Viruses counter this defense by expressing viral suppressors of RNAi (VSRs). While VSRs in Drosophila melanogaster were shown to inhibit RNAi through different modes of action, whether they act on other silencing pathways remained unexplored. Here we show that expression of various plant and insect VSRs in transgenic flies does not perturb the Drosophila microRNA (miRNA) pathway; but in contrast, inhibits antiviral RNAi and the RNA silencing response triggered by inverted repeat transcripts, and injection of dsRNA or siRNA. Strikingly, these VSRs also suppressed transposon silencing by endogenous siRNAs (endo-siRNAs). Our findings identify VSRs as tools to unravel small RNA pathways in insects and suggest a cosuppression of antiviral RNAi and endo-siRNA silencing by viruses during fly infections.