Distinguishing molecular forms of HIV-1 in Asia with a high-throughput, fluorescent genotyping assay, MHAbce v.2
Distinguishing molecular forms of HIV-1 in Asia with a high-throughput, fluorescent genotyping assay, MHAbce v.2
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DOI：
10.1016/j.virol.2006.07.055
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发表时间：
2007-02-05
期刊：
影响因子：
3.7
通讯作者：
McCutchan, Francine E.
中科院分区：
文献类型：
--
作者：
Kijak, Gustavo H.;Tovanabutra, Sodsai;McCutchan, Francine E.
大样本组的高分辨率HIV-1基因分型对于确定亚洲不断演变和动态的流行病至关重要。在此，我们介绍了MHAbce v.2，这是一种多区域杂交试验，基于实时PCR，在病毒基因组的8个区域使用亚型特异性TaqMan探针，可以单独区分B、C、CRF01-AE亚型以及几乎所有描述的重组体。在验证组（n = 70）中，该分析的灵敏度为95.7%，特异性为99.8%。对回顾性MTCT队列（n = 180；南邦省，泰国北部；1996-1998年）的样本进行了现场检测。177/180份样本可分型，其中94.4%为CRF01-AE。剩余的菌株代表了B亚型和B/CRF01-AE重组的均匀比例，并通过测序得到证实，揭示了泰国异性恋和IDU HIV-1流行之间的早期联系。MHAbce v.2的适用地区包括中国、印度、东南亚和环太平洋地区，可用于全面和详细地了解艾滋病毒/艾滋病流行病的这一重要组成部分。Elsevier Inc.出版。
High-resolution HIV-1 genotyping of large sample sets is crucial to define the evolving and dynamic epidemics in Asia. Here we present MHAbce v.2, a multi-region hybridization assay that individually discriminates subtypes B, C, CRF01-AE, and virtually all of their described recombinants, based on real-time PCR using subtype-specific TaqMan probes in 8 regions throughout the viral genome. In a validation panel (n = 70), the assay performed with a sensitivity of 95.7% and specificity of 99.8%. The assay was field-tested on samples from a retrospective MTCT cohort (n = 180; Lampang Province, Northern Thailand; 1996-1998). 177/180 of the samples were typeable, and 94.4% were typed as CRF01-AE. The remaining strains represented even proportions of subtype B and B/CRF01-AE recombinants and were confirmed by sequencing, revealing early links between the heterosexual and IDU HIV-1 epidemics in Thailand. MHAbce v.2, with an area of application including China, India, Southeast Asia, and the Pacific Rim, can be used to develop a comprehensive and detailed picture of this important component of the HIV/AIDS pandemic. Published by Elsevier Inc.