An automated two-phase microfluidic system for kinetic analyses and the screening of compound libraries
An automated two-phase microfluidic system for kinetic analyses and the screening of compound libraries
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DOI：
10.1039/b921754a
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发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Merten, Christoph A.
中科院分区：
文献类型：
--
作者：
Clausell-Tormos, Jenifer;Griffiths, Andrew D.;Merten, Christoph A.
基于液滴的微流控系统使生物和化学反应的规模大大降低。然而，将外部世界与这类系统连接并产生大量不同化学成分的微滴仍然具有挑战性。我们在这里描述了一种自动化系统，其中从微量滴定板产生化学上不同的塞子阵列。每个阵列可以拆分成多个小容量副本，从而允许同一存储库的几个屏幕。该系统完全兼容进一步的片上操作(S)，并允许随着时间的推移监控单个塞子(例如，记录反应动力学)。因此，该技术消除了目前基于液滴的微流控系统的几个瓶颈，并应为(生物)化学和基于细胞的筛选开辟道路。
Droplet-based microfluidic systems allow biological and chemical reactions to be performed on a drastically decreased scale. However, interfacing the outside world with such systems and generating high numbers of microdroplets of distinct chemical composition remain challenging. We describe here an automated system in which arrays of chemically distinct plugs are generated from microtiter plates. Each array can be split into multiple small-volume copies, thus allowing several screens of the same library. The system is fully compatible with further on-chip manipulation(s) and allows monitoring of individual plugs over time (e. g. for recording reaction kinetics). Hence the technology eliminates several bottlenecks of current droplet-based microfluidic systems and should open the way for (bio-)chemical and cell-based screens.