A novel long noncoding RNA OECC promotes colorectal cancer development and is negatively regulated by miR-143-3p
A novel long noncoding RNA OECC promotes colorectal cancer development and is negatively regulated by miR-143-3p
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一种新型长非编码RNA OECC促进结直肠癌的发展并受到miR-143-3p的负调控
DOI：
10.1016/j.bbrc.2018.08.075
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发表时间：
2018-09-18
影响因子：
3.1
通讯作者：
Liu, Huanliang
中科院分区：
文献类型：
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作者：
Huang, Fengting;Wen, Chuangyu;Liu, Huanliang
新出现的证据表明，异常的长非编码RNA（lncRNA）表达有助于CRC发病机制。为了探讨IncRNA在结直肠癌中的生物学功能并确定其潜在机制，我们首先进行了IncRNA微阵列分析以研究IncRNA在结直肠癌中的表达模式。我们发现了一种新的lncRNA OECC，起源于染色体8 q24，在CRC组织和细胞系中高度表达，并与肝转移呈正相关。IncRNA OECC表达的减弱抑制CRC细胞增殖，诱导凋亡，并抑制迁移。此外，观察到lncRNA OECC和miR-143- 3 p之间的负相关性。生物信息学分析预测，荧光素酶报告基因测定证明，IncRNA OECC是miR-143- 3 p的直接靶点，导致其靶基因NF-κ B和p38 MAPK途径表达下调。综上所述，我们的研究结果表明IncRNA OECC在CRC中过表达，并且可能通过miR-143- 3 p通过NF-κ B和p38 MAPK通路激活发挥致癌作用。(C)2018作者爱思唯尔公司出版
Emerging evidence indicates that aberrant long non-coding RNA (lncRNA) expression contributes to CRC pathogenesis. To explore the biological functions of IncRNAs in CRC and to identify the underlying mechanisms, we first conducted a IncRNA microarray assay to investigate lncRNA expression patterns in CRC. We identified a novel lncRNA OECC, originating from chromosome 8q24 that is highly expressed in CRC tissues and cell lines and has a positive correlation with liver metastasis. Attenuation of IncRNA OECC expression prohibited CRC cell proliferation, induced apoptosis, and inhibited migration. Furthermore, an inverse correlation between lncRNA OECC and miR-143-3p was observed. Bioinformatic analyses predicted, and a luciferase reporter assay demonstrated, that IncRNA OECC is a direct target of miR-143-3p, leading to down-regulation in the expression of its target genes, the NF-kappa B and p38 MAPK pathways. Taken together, our results suggest that IncRNA OECC is overexpressed in CRC and may play an oncogenic role through NF-kappa B and p38 MAPK pathway activation via miR-143-3p. (C) 2018 The Authors. Published by Elsevier Inc.