Regulation of insect steroid hormone biosynthesis by innervating peptidergic neurons.
Regulation of insect steroid hormone biosynthesis by innervating peptidergic neurons.
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DOI：
10.1073/pnas.0511196103
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发表时间：
2006-06
影响因子：
11.1
通讯作者：
N. Yamanaka;D. Z̆itn̆an;Young-Joon Kim;M. Adams;Y. Hua;Yusuke Suzuki;Minoru Suzuki;A. Suzuki;H. Satake;A. Mizoguchi;K. Asaoka;Yoshiaki Tanaka;H. Kataoka
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文献类型：
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作者：
N. Yamanaka;D. Z̆itn̆an;Young-Joon Kim;M. Adams;Y. Hua;Yusuke Suzuki;Minoru Suzuki;A. Suzuki;H. Satake;A. Mizoguchi;K. Asaoka;Yoshiaki Tanaka;H. Kataoka
在昆虫中，被称为蜕皮激素的类固醇激素引起蜕皮和变态。前胸腺（PG）是体外甾体激素的主要来源，其生物合成（体外甾体生成）受几种神经肽的调节。在这里，我们报道了fmrfamily相关肽（FaRPs）通过家蚕PG的直接神经支配来调节卵巢甾体形成。我们纯化了一个以前未被鉴定的家蚕FaRP， DPSFIRFamide，并鉴定了相应的家蚕FMRFamide基因（Bommo-FMRFamide, BRFa），该基因编码另外三个FaRP。所有BRFa肽都通过Bommo-myosuppressin受体减少cAMP的产生来抑制PG中的外甾体生成，Bommo-myosuppressin是另一种FaRP，我们之前已经证明它是一种促胸抑压因子。BRFa主要在胸神经节的神经分泌细胞中表达，胸前神经节的神经元支配PG向腺体表面提供所有四种多肽。发育过程中的电生理记录证实，在PG活性较低的阶段，BRFa神经元的放电活性增加，血淋巴中外皮甾体水平降低。据我们所知，这项研究首次报道了肽通过直接神经支配控制卵巢甾体生成。
In insects, steroid hormones named ecdysteroids elicit molting and metamorphosis. The prothoracic gland (PG) is a predominant source of ecdysteroids, where their biosynthesis (ecdysteroidogenesis) is regulated by several neuropeptides. Here, we report that FMRFamide-related peptides (FaRPs) regulate ecdysteroidogenesis through direct innervation of the PG in the silkworm Bombyx mori. We purified a previously uncharacterized Bombyx FaRP, DPSFIRFamide, and identified the corresponding Bombyx FMRFamide gene (Bommo-FMRFamide, BRFa), which encodes three additional FaRPs. All BRFa peptides suppressed ecdysteroidogenesis in the PG by reducing cAMP production by means of the receptor for Bommo-myosuppressin, another FaRP we have previously shown to act as a prothoracicostatic factor. BRFa is predominantly expressed in neurosecretory cells of thoracic ganglia, and the neurons in the prothoracic ganglion innervate the PG to supply all four peptides to the gland surface. Electrophysiological recordings during development confirmed the increased firing activity of BRFa neurons in stages with low PG activity and decreased ecdysteroid levels in the hemolymph. To our knowledge, this study provides the first report of peptides controlling ecdysteroidogenesis by direct innervation.