Induction of opsonic antibodies to Pseudomonas aeruginosa mucoid exopolysaccharide by an anti-idiotypic monoclonal antibody.
Induction of opsonic antibodies to Pseudomonas aeruginosa mucoid exopolysaccharide by an anti-idiotypic monoclonal antibody.
复制标题
通过抗独特型单克隆抗体诱导针对铜绿假单胞菌粘液胞外多糖的调理性抗体。
DOI：
10.1093/infdis/164.3.507
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Patawaran,M
中科院分区：
文献类型：
--
作者：
Schreiber,JR;Pier,GB;Grout,M;Nixon,K;Patawaran,M
铜绿假单胞菌的粘液型菌株是囊性纤维化患者的主要肺部病原体。粘液外多糖（MEP）抗原的调理抗体可以保护动物和一些囊性纤维化患者免受感染。然而，MEP在慢性感染期间或接种疫苗后不容易引起调理素抗体。为了评估诱导调理素抗体的替代方法，产生了针对MEP特异性调理素单克隆抗体的鼠单克隆抗独特型抗体。抗独特型抗体与调理素抗体的F（ab '）2片段结合，阻断与MEP的结合，与针对MEP的人调理素抗体上的交叉反应性独特位结合，并在同基因和同种异体小鼠中引发MEP特异性抗体。这些抗独特型诱导的MEP特异性抗体将补体固定到mucoidP。aerobiosacell并调理它们以用于人白细胞的吞噬杀伤。这些研究表明，抗独特型单克隆抗体的潜在效用产生保护性免疫对细菌多糖。
Mucoid strains ofPseudomonas aeruginosaare the major pulmonary pathogens for cystic fibrosis patients. Opsonizing antibodies to the mucoid exopolysaccharide (MEP) antigen may protect animals and some cystic fibrosis patients from infection. However, MEP does not readily elicit opsonic antibodies either during chronic infection or after vaccination. To evaluate alternative means to induce opsonic antibodies, a murine monoclonal anti-idiotypic antibody directed to an opsonic monoclonal antibody specific to MEP was produced. The anti-idiotypic antibody bound to F(ab')2fragments of the opsonic antibody, blocked binding to MEP, bound to crossreactive idiotopes on human opsonic antibodies to MEP, and elicited MEP-specific antibodies in syngeneic and allogeneic mice. These anti-idiotype-induced, MEP-specific antibodies fixed complement to mucoidP. aeruginosacells and opsonized them for phagocytic killing by human leukocytes. These studies demonstrate the potential utility of anti-idiotypic monoclonal antibody for generating protective immunity against bacterial polysaccharides.