The Arabidopsis thaliana MND1 homologue plays a key role in meiotic homologous pairing, synapsis and recombination
The Arabidopsis thaliana MND1 homologue plays a key role in meiotic homologous pairing, synapsis and recombination
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DOI：
10.1242/jcs.02967
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发表时间：
2006-06-15
影响因子：
4
通讯作者：
Schloegelhofer, Peter
中科院分区：
文献类型：
--
作者：
Kerzendorfer, Claudia;Vignard, Julien;Schloegelhofer, Peter
最近在酵母中发现了Mnd1在减数分裂重组中的关键作用。在这里，我们描述了拟南芥同源基因AtMND1的鉴定和功能特性，AtMND1对雄性和雌性减数分裂是必不可少的，从而对植物的育性也是必不可少的。在突变植株中，虽然轴向元件正常形成，姐妹染色单体凝聚力已经建立，重组起始似乎不受影响，但染色体不会突触。在减数分裂过程中，观察到大量由染色质桥相互连接的缠绕的染色体，并出现严重的染色体断裂。这些缺陷取决于SPO11-1的存在，SPO11-1是一种通过催化DNA双链断裂(DSB)形成而启动重组的蛋白质。此外，我们证明了AtMND1蛋白与AHP2相互作用，AHP2是拟南芥中与萌芽酵母Hop2密切相关的蛋白。这些数据表明，AtMND1在同源突触和减数分裂重组过程中的DSB修复中发挥着关键作用。
Mnd1 has recently been identified in yeast as a key player in meiotic recombination. Here we describe the identification and functional characterisation of the Arabidopsis homologue, AtMND1, which is essential for male and female meiosis and thus for plant fertility. Although axial elements are formed normally, sister chromatid cohesion is established and recombination initiation appears to be unaffected in mutant plants, chromosomes do not synapse. During meiotic progression, a mass of entangled chromosomes, interconnected by chromatin bridges, and severe chromosome fragmentation are observed. These defects depend on the presence of SPO11-1, a protein that initiates recombination by catalysing DNA double-strand break (DSB) formation. Furthermore, we demonstrate that the AtMND1 protein interacts with AHP2, the Arabidopsis protein closely related to budding yeast Hop2. These data demonstrate that AtMND1 plays a key role in homologous synapsis and in DSB repair during meiotic recombination.