Sustained gastrointestinal colonization and systemic dissemination by Candida albicans, Candida tropicalis and Candida parapsilosis in adult mice
Sustained gastrointestinal colonization and systemic dissemination by Candida albicans, Candida tropicalis and Candida parapsilosis in adult mice
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DOI：
10.1016/s0732-8893(00)00165-6
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发表时间：
2000-09-01
影响因子：
2.9
通讯作者：
Rodríguez-Tudela, JL
中科院分区：
文献类型：
--
作者：
Mellado, E;Cuenca-Estrella, M;Rodríguez-Tudela, JL
测定了九种临床分离的念珠菌（三种白色念珠菌、三种热带念珠菌和三种近平滑念珠菌）定植和侵入成年雄性 CD-1 OCR 小鼠胃肠道的能力。评估了膳食四环素加葡萄糖补充剂对定植的影响。将菌株胃内接种。四环素和葡萄糖显着改变了需氧菌群，尤其是链球菌（平均下降 1.1 +/- 0.3 log(10) CFU/g，根据学生 t 检验，p < 0.01）。口服攻击后两周，所有接受葡萄糖-四环素补充剂的小鼠均实现了念珠菌在胃肠道的持续和高定植（平均 5.28 +/- 0.18 log(10) CFU/g，p < 0.01），接种了一种热带念珠菌分离株的动物除外。胃的组织学切片显示心房皱襞区域有多个与菌丝相关的上皮内微脓肿。在免疫抑制下，接受膳食补充剂的动物中分别有 62% 和 24% 观察到白色念珠菌和热带念珠菌的全身传播。近平滑念珠菌分离株没有发现传播。我们开发了一种简单且廉价的胃肠道持续定植小鼠模型。该模型可用于分析系统性念珠菌感染的预防、治疗和诊断以及评估菌株的毒力。 (C) 2000 Elsevier Science Inc. 保留所有权利。
The ability of nine clinical isolates of Candida species (three C. albicans, three C. tropicalis and three C. parapsilosis) to colonize and invade the gastrointestinal (GI) tract of adult male CD-1 OCR) mice was determined. The effect of dietary tetracycline plus glucose supplementation on colonization was evaluated. Strains were intragastrically inoculated. Tetracycline and glucose altered substantially aerobic flora, especially streptococci (average fall 1.1 +/- 0.3 log(10) CFU/g, p < 0.01 by the Student's t test). At two weeks after oral challenge, sustained and high colonization of GI tract by Candida (mean 5,28 +/- 0.18 log(10) CFU/g, p < 0.01) was achieved in all mice receiving glucose-tetracycline supplementation, excepting in animals inoculated with one of C. tropicalis isolates. Histologic sections of the stomachs revealed multiple intraepithelial micro-abscesses associated with hyphae in the region of the cardial-atrium fold. Under immunosuppression, systemic spread of C. albicnns and C. tropicalis was observed in 62% and 24% of animals receiving dietary supplementation respectively. Dissemination was not noted for C. parapsilosis isolates. We have developed a simple and inexpensive murine model of sustained colonization of GI tract. This model could be useful for analyzing prophylaxis, treatment and diagnosis of systemic Candida infections and for evaluating virulence of strains. (C) 2000 Elsevier Science Inc. All rights reserved.