Structural characterization of low molecular weight polysaccharide from Astragalus membranaceus and its immunologic enhancement in recombinant protein vaccine against systemic candidiasis
Structural characterization of low molecular weight polysaccharide from Astragalus membranaceus and its immunologic enhancement in recombinant protein vaccine against systemic candidiasis
复制标题
黄芪低分子量多糖的结构表征及其在抗系统性念珠菌病重组蛋白疫苗中的免疫增强作用
DOI：
10.1016/j.carbpol.2016.03.024
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发表时间：
2016
影响因子：
11.2
通讯作者：
Wang Guiyun
中科院分区：
文献类型：
--
作者：
Yang Fan;Xiao Chunyu;Qu Jing;Wang Guiyun
黄芪低分子量多糖的结构及其免疫增强作用在重组蛋白疫苗中检测到。LMW-ASP的结构分析表明，LMW-ASP（分子量为5.6 kDa）是一种酸性杂多糖，由Glc、Gal、Ara、Xyl和GalA组成，其比例为10.0：1.3：1.7：1.0：0.9。以白念珠菌热休克蛋白90（HSP 90）C表位（LKVIRK）重组蛋白（rP-HSP 90 C）为疫苗。结果表明，LMW-ASP能显著提高rP-HSP 90 C免疫小鼠血清中IgG、IgG 1、IgG 2b和IL-2、IL-4、IL-10、IL-12的特异性抗体滴度（p< 0.05）。LMW-ASP还能增强HSP 90 C诱导的小鼠DTH反应。更重要的是，与用rP-HSP 90 C免疫的小鼠相比，用rP-HSP 90 C/LMW-ASP免疫的小鼠在肾脏中具有更少的CFU（集落形成单位）（p< 0.05）。因此，LMW-ASP可作为一种新型佐剂，用于增强重组蛋白疫苗的免疫效果。
Structure and immunologic enhancement of low molecular weight polysaccharide (LMW-ASP) isolated from the root ofAstragalus membranaceus(Fisch) Bge. Were detected in recombinant protein vaccine. Structure analysis of LMW-ASP revealed that LMW-ASP (Mw= 5.6 kDa) was an acid heteropolysaccharide, which consisted of Glc, Gal, Ara, Xyl and GalA in ratio of 10.0:1.3:1.7:1.0:0.9. Recombinant protein (rP-HSP90C) contained epitope C (LKVIRK) from heat shock protein 90 (HSP90) ofCandida albicanswas used as a vaccine. The results indicated that LMW-ASP significantly promoted specific antibody titers IgG, IgG1, IgG2b, and IL-2, IL-4, IL-10, IL-12 in sera of mice immunized with rP-HSP90C (p< 0.05). It was also found LMW-ASP improved DTH response in HSP90C-injceted mice. More importantly, the mice immunized with rP-HSP90C/LMW-ASP had fewer CFU (colony forming unites) in the kidneys compared to the mice immunized with rP-HSP90C (p< 0.05). Therefore, LMW-ASP could be exploited into the novel adjuvant to enhance the efficacy of recombinant protein vaccine.