Reduction of Hg2+ with Reduced Mammalian Cytochrome c by Cytochrome c Oxidase Purified from a Mercury-Resistant Acidithiobacillus ferrooxidans Strain, MON-1
Reduction of Hg2+ with Reduced Mammalian Cytochrome c by Cytochrome c Oxidase Purified from a Mercury-Resistant Acidithiobacillus ferrooxidans Strain, MON-1
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从耐汞氧化亚铁硫杆菌菌株 MON-1 中纯化的细胞色素 c 氧化酶对 Hg2 进行还原，从而还原哺乳动物细胞色素 c
DOI：
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
F. Takeuchi
中科院分区：
文献类型：
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作者：
T. Sugio;M. Fujii;Y. Ninomiya;T. Kanao;A. Negishi;F. Takeuchi
嗜酸氧化亚铁硫杆菌AP 19 -3、ATCC 23270和MON-1分别是汞敏感、中度耐汞和高度耐汞菌株。已知2，3，5，6-四甲基-对苯二胺（TMPD）和还原的细胞色素c用作细胞色素c氧化酶特异性的电子供体。菌株MON-1的静息细胞具有TMPD氧化酶活性，并以TMPD为电子供体挥发金属汞。从菌株MON-1纯化的细胞色素c氧化酶还原汞离子与还原哺乳动物细胞色素c以及TMPD金属汞。这些汞挥发活动减少细胞色素c和TMPD完全抑制1 mM NaCN。这些结果表明，细胞色素c氧化酶参与了A.氧化亚铁细胞菌株AP 19 -3和ATCC 23270的细胞色素c氧化酶活性分别被1 μM和5 μM氯化汞完全抑制。而MON-1菌株的活性在5 μM和10 μM氯化汞浓度下分别被抑制33%和70%。ferrooxidans菌株与细胞色素c氧化酶的汞抗性水平很好地对应。
Acidithiobacillus ferrooxidans AP19-3, ATCC 23270, and MON-1 are mercury-sensitive, moderately mercury-resistant, and highly mercury-resistant strains respectively. It is known that 2,3,5,6-tetramethyl-p-phenylendiamine (TMPD) and reduced cytochrome c are used as electron donors specific for cytochrome c oxidase. Resting cells of strain MON-1 had TMPD oxidase activity and volatilized metal mercury with TMPD as an electron donor. Cytochrome c oxidase purified from strain MON-1 reduced mercuric ions to metalic mercury with reduced mammalian cytochrome c as well as TMPD. These mercury volatilization activities with reduced cytochrome c and TMPD were completely inhibited by 1 mM NaCN. These results indicate that cytochrome c oxidase is involved in mercury reduction in A. ferrooxidans cells. The cytochrome c oxidase activities of strains AP19-3 and ATCC 23270 were completely inhibited by 1 μM and 5 μM of mercuric chloride respectively. In contrast, the activity of strain MON-1 was inhibited 33% by 5 μM, and 70% by 10 μM of mercuric chloride, suggesting that the levels of mercury resistance in A. ferrooxidans strains correspond well with the levels of mercury resistance of cytochrome c oxidase.
影响因子：
10.5
作者：
A. Summers
通讯作者：
A. Summers