NUCLEOTIDE-SEQUENCE OF THE GLYCOPROTEIN-S GENE OF BOVINE ENTERIC CORONAVIRUS AND COMPARISON WITH THE S-PROTEINS OF 2 MOUSE HEPATITIS-VIRUS STRAINS
NUCLEOTIDE-SEQUENCE OF THE GLYCOPROTEIN-S GENE OF BOVINE ENTERIC CORONAVIRUS AND COMPARISON WITH THE S-PROTEINS OF 2 MOUSE HEPATITIS-VIRUS STRAINS
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DOI：
10.1099/0022-1317-71-2-487
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发表时间：
1990-02-01
影响因子：
3.8
通讯作者：
LAPORTE, J
中科院分区：
文献类型：
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作者：
BOIREAU, P;CRUCIERE, C;LAPORTE, J
克隆了牛肠道冠状病毒(BECV)S蛋白(S)基因，测定了其4092个核苷酸的全序列。该序列含有一个长的开放阅读框，编码1363个氨基酸(Mr 150747)。预测的蛋白质有19个N-糖基化位点。从第一个蛋氨酸残基开始，观察到一个由17个氨基酸组成的信号序列。在氨基酸763和767之间有一个潜在的肽酶切割位点。这些裂解解释了S基因初级产物成熟为S1(MR 104692)和S2(MR 84175)刺突结构蛋白的原因。还观察到两个两亲性α-螺旋(氨基酸1007到1077和1269到1294)，它们可能构成病毒尖峰的12 nm柄；另一个α-螺旋(氨基酸1305到1335)可能参与病毒膜中尖峰的锚定。将该蛋白序列与已报道的同源小鼠肝炎病毒A59株和S株的同源蛋白序列进行比较，我们认为S株和A59株的同源蛋白序列可能来源于S基因的缺失。
The gene encoding the spike glycoprotein (S) of bovine enteric coronavirus (BECV) was cloned and its complete sequence of 4092 nucleotides was determined. This sequence contained a single long open reading frame with a coding capacity of 1363 amino acids (Mr 150747). The predicted protein had 19 N-glycosylation sites. A signal sequence comprising 17 amino acids was observed starting from the first methionine residue. A potential peptidase cleavage site was located between amino acids 763 and 767. These cleavages explain the maturation of the primary product of the S gene to S1 (Mr 104692) and S2 (Mr 84175) spike structural proteins. Two amphipathic .alpha.-helices (amino acids 1007 to 1077 and 1269 to 1294) which may constitute the 12 nm stalk of the viral spike were also observed; another .alpha.-helix (amino acids 1305 to 1335) may be involved in the anchorage of the spike in the viral membrane. Comparison of this protein sequence to the described homologous mouse hepatitis (MHV) strain A59 and MHV-JHM S protein sequences led us to suggest that MHV-A59 and MHV-JHM S genes could be derived from a deletion of the BECV S gene.