Phenotype and function of rat dendritic cell subsets
Phenotype and function of rat dendritic cell subsets
复制标题
大鼠树突状细胞亚群的表型和功能
DOI：
10.1034/j.1600-0463.2003.11107807.x
复制
发表时间：
2003
期刊：
影响因子：
2.8
通讯作者：
G. Macpherson
中科院分区：
文献类型：
--
作者：
U. Yrlid;G. Macpherson
树突状细胞（DC）包括表型不同的亚群，其在免疫诱导中具有不同的功能。了解体内DC亚群的生物学对于理解疾病中的免疫调节及其扰动至关重要。本文综述了大鼠DC亚群的表型和功能，并与其他物种中鉴定的亚群进行了比较。我们的研究集中在DC在淋巴中的迁移。DC组成性地从外周组织迁移到引流节点，可能是为了诱导/维持对自身或无害的外来抗原的耐受性。在大鼠中去除肠系膜淋巴结（MLN）后，传入和传出淋巴管的愈合允许从胸导管收集迁移的肠DC（iLDC）。我们已经表明，iLDC包括至少两个子集，不同的表型，原位分布和功能。CD 4 +/SIRPα+ iLDC具有高度免疫刺激性，但被排除在MLN的T细胞区域之外。相比之下，CD 4 −/SIRPα− iLDC对T细胞的刺激作用较弱，但可将凋亡肠上皮细胞的物质携带至MLN的T细胞区域。淋巴结和脾脏中存在类似的亚群。研究表明，表型相似的亚群在牛和羊的皮肤引流淋巴中迁移。我们和其他人已经表明，脾脏CD 4 −/SIRPα− DC可以在体外吞噬同种异体细胞，是CD 8 + T细胞的弱刺激因子，并且可以裂解NK敏感性靶细胞。尽管我们的一些数据表明大鼠CD 4 −/SIRPα− DC可能等同于小鼠CD 8 + DC，但目前还没有足够的证据对此有信心。
Dendritic cells (DC) comprise phenotypically‐distinct subsets that sub‐serve distinct functions in immune induction. Understanding the biology of DC subsets in vivo is crucial for the understanding of immune regulation and its perturbations in disease. This review focuses on the phenotype and functions of rat DC subsets and compares these with subsets identified in other species. Our research has concentrated on DC migrating in lymph. DC migrate constitutively from peripheral tissues to draining nodes, probably to induce/maintain tolerance to self‐ or harmless foreign antigens. After removal of mesenteric lymph nodes (MLN) in the rat, healing of afferent and efferent lymphatics permits migrating intestinal DC (iLDC) to be collected from the thoracic duct. We have shown that iLDC consist of least two subsets that differ in phenotype, in situ distribution and function. CD4+/SIRPα+ iLDC are highly immunostimulatory, but are excluded from T cell areas of MLN. In contrast, CD4−/SIRPα− iLDC are less potent stimulators of T cells, but carry material from apoptotic enterocytes to T cell areas of MLN. Similar subsets exist in both lymph nodes and spleen. It has been shown that phenotypically‐similar subsets migrate in skin‐draining lymph in cattle and sheep. We and others have shown that splenic CD4−/SIRPα− DC can phagocytose allogeneic cells in vitro, are poor stimulators of CD8+ T cells, and can lyse NK‐sensitive target cells. Although some of our data suggest that rat CD4−/SIRPα− DC may equate to murine CD8+ DC, there is at present insufficient evidence to be confident of this.