Cross-clade CD8 T-cell responses to HIVIIIB and Chinese B′ and C/B′ viruses in North American and Chinese HIV-seropositive donors
Cross-clade CD8 T-cell responses to HIVIIIB and Chinese B′ and C/B′ viruses in North American and Chinese HIV-seropositive donors
复制标题
DOI：
10.1097/01.qai.0000145220.81304.b0
复制
发表时间：
2004-12-01
影响因子：
3.6
通讯作者：
Lieberman, J
中科院分区：
文献类型：
--
作者：
François-Bongarcon, V;Feng, Y;Lieberman, J
HIV变异对全球艾滋病疫苗的研发构成了障碍。病毒多样性和宿主 MHC 表达变异都会影响疫苗反应。来自世界某一地区的 HIV 感染者的 CD8 T 细胞是否能够交叉识别来自其他地区的分离株，将为是否需要针对特定国家的疫苗提供指导。我们比较了 7 名感染 HIV 的北美捐献者和 4 名中国捐献者的 CD8 T 细胞对 HIVIIIB 和中国流行的代表性 B'（泰国 B）和重组 C/B' 病毒株的识别。针对 HIV、、、或中国病毒的 IFN-γ 产生具有可比性。尽管1.6 +/- 0.8%的美国供体CD8 T细胞产生高于背景水平的IFN-γ来响应IIIB病毒，但1.5 +/- 0.8%响应B'病毒，1.4 +/- 0.7%响应C/B'病毒。对感染表达B'和C/B'病毒gag和env的痘苗病毒的贴壁细胞的反应在强度上也与对IIIB病毒的反应相当。感染 B' 病毒的 CD4 T 细胞的细胞裂解与感染 IIIB 病毒的细胞的裂解相当，但差异更大的 C/B' 病毒的裂解有所减少。被选择用于产生针对 IIIB 病毒的 IFN-γ 的 T 细胞，也能有效裂解感染中国病毒的细胞。因此，针对 IIIB 病毒和中国流行病毒株的跨进化枝 CD8 T 细胞反应很常见。
HIV variation presents an obstacle to a global AIDS vaccine. Viral diversity and host variations in MHC expression both affect vaccine responses. Whether CD8 T cells from HIV-infected donors in 1 part of the world cross-recognize isolates from other regions will provide guidance about whether country-specific vaccines are needed. We compared recognition of HIVIIIB and representative B' (Thai B) and recombinant C/B' virus strains endemic in China by CD8 T cells from 7 HIV-infected North American donors and 4 Chinese donors. IFN-gamma production in response to HIV,,,, or the Chinese viruses was comparable. Although 1.6 +/- 0.8% of American donor CD8 T cells produced IFN-gamma above the background level in response to IIIB virus, 1.5 +/- 0.8% responded to B' virus, and 1.4 +/- 0.7% responded to C/B' vir-us. Responses to adherent cells infected with vaccinia viruses expressing B' and C/B' virus gag and env were also comparable in magnitude with responses to IIIB virus. Cytolysis of CD4 T cells infected with B' virus was comparable with lysis of cells infected with IIIB virus, but lysis of the more divergent C/B' virus was somewhat reduced. T cells, selected for IFN-gamma production to IIIB virus, also efficiently lysed cells infected with Chinese viruses. Therefore, cross-clade CD8 T-cell responses to IIIB virus and prevalent Chinese viral strains are common.