ACCELERATION OF GROWTH OF CULTURED CARDIOMYOCYTES AND TRANSLOCATION ON OF PROTEIN-KINASE-C
ACCELERATION OF GROWTH OF CULTURED CARDIOMYOCYTES AND TRANSLOCATION ON OF PROTEIN-KINASE-C
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DOI：
10.1152/ajpcell.1992.263.2.c319
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发表时间：
1992-08-01
影响因子：
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通讯作者：
MORGAN, HE
中科院分区：
文献类型：
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作者：
ALLO, SN;CARL, LL;MORGAN, HE
佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）、去甲肾上腺素（NE）和收缩刺激心肌细胞生长（增加蛋白质含量）。蛋白质和RNA积累的时间进程和程度存在差异。以4 × 10（6）个细胞/60 mm培养皿接种并用50 mM KCl阻滞的细胞显示无显著生长。PMA处理48 h后，生长量最高可达17%。相比之下，收缩和NE处理的细胞的最大生长刺激分别为48小时的36%和72小时的31%。PMA处理的细胞在24小时对蛋白质合成能力（RNA含量）的最大刺激为32%，而NE处理的细胞和收缩细胞在72小时分别为59%和77%。为了支持改变能力在调节蛋白质合成中的主要作用，RNA和蛋白质含量之间存在显着相关性（r = 0.84），且与所使用的刺激无关。血管紧张素II在48 h时使RNA含量增加28%，但在72 h内对生长没有影响。生长刺激和增加核蛋白激酶C（PKC）的活性诱导收缩，NE，和PMA治疗和抑制staurosporine（PKC抑制剂），提示PKC的核心作用。
Phorbol 12-myristate 13-acetate (PMA), norepinephrine (NE), and contraction stimulate cardiomyocyte growth (increased protein content). Differences exist in the time course and extent of protein and RNA accumulation. Cells plated at 4 x 10(6) cells/60-mm dish and arrested with 50 mM KCl demonstrated no significant growth. Treatment with PMA stimulated growth to a maximum of 17% at 48 h. In contrast, maximal stimulation of growth was 36 % at 48 h and 31 % at 72 h for contracting and NE-treated cells, respectively. Maximal stimulation of the capacity for protein synthesis (RNA content) was 32% for PMA-treated cells at 24 h compared with 59% and 77% for NE-treated and contracting cells, respectively, at 72 h. In support of a primary role for altered capacity in the regulation of protein synthesis, there was a significant correlation (r = 0.84) between RNA and protein contents that was independent of the stimulus used. Angiotensin II increased RNA content by 28% at 48 h but had no effect on growth up to 72 h. Growth stimulation and increased nuclear protein kinase C (PKC) activity were induced by contraction, NE, and PMA treatment and were inhibited by staurosporine (a PKC inhibitor), suggestive of a central role for PKC.