Gamma/delta T cells from tolerized alpha/beta-TCR-deficient mice antigen specifically inhibit contact sensitivity in vivo and IFN-gamma production in vitro.
Gamma/delta T cells from tolerized alpha/beta-TCR-deficient mice antigen specifically inhibit contact sensitivity in vivo and IFN-gamma production in vitro.
复制标题
来自耐受的 α/β-TCR 缺陷小鼠抗原的 γ/δ T 细胞特异性抑制体内接触敏感性和体外 IFN-γ 产生。
DOI：
10.1159/000237607
复制
发表时间：
1997
影响因子：
2.8
通讯作者：
Askenase,PW
中科院分区：
文献类型：
--
作者：
Szczepanik,M;Anderson,LR;Ushio,H;Ptak,W;Owen,MJ;Hayday,AC;Askenase,PW
对反应性半抗原（Ag）（如苦基氯）的接触敏感性（CS）应答是体内T细胞介导的免疫应答的经典实例。还有大量证据表明，体内暴露于高剂量静脉内Ag的T细胞可以下调CS（高剂量Ag耐受性）。为了阐明影响CS并介导其下调的细胞类型，我们研究了先天性α/β T细胞缺陷小鼠（α-/-小鼠）的CS。我们发现，α-7-小鼠不能安装CS，暗示α/β T细胞作为关键的CS效应细胞。然而，在高剂量Ag耐受后，这些α-7-小鼠可以下调过继转移到它们的α/β CS效应细胞。耐受化α-/-小鼠中的活性细胞是γ/δ TCR+细胞，其在过继细胞转移后特异性下调CS效应子α/β T细胞Ag。此外，γ/δ细胞可以在体外特异性下调CS效应细胞产生IFN-γ。这些发现证实γ/δ T细胞不是CS效应细胞，但下调CS，与最近报道的γ/δ T细胞下调IgE应答一致。
Contact sensitivity (CS) responses to reactive hapten antigens (Ag), such as picryl chloride, are classical examples of T-cell-mediated immune responses in vivo. There is also abundant evidence that T cells exposed in vivo to high intravenous doses of Ag can downregulate CS (high-dose Ag tolerance). To clarify cell types that effect CS and mediate its downregulation, we have studied CS in mice congenitally deficient in α/β T cells (α-/- mice). We show that α-7- mice cannot mount CS, implicating α/β T cells as critical CS effector cells. However, after high-dose Ag tolerization, these α-7- mice can downregulate α/β CS effector cells adoptively transferred to them. The active cells in tolerized α-/- mice are γ/δ TCR+ cells which downregulate CS effector α/β T cells Ag-specifically upon adoptive cell transfer. Moreover, γ/δ cells can Ag-specifícally downregulate IFN-γ production by CS effector cells in vitro. These findings establish that γ/δ T cells are not CS effector cells but downregulate CS, in agreement with recent reports that γ/δ T cells downregulate IgE responses.