Prosaposin in the rat oviductal epithelial cells
Prosaposin in the rat oviductal epithelial cells
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大鼠输卵管上皮细胞中的前列腺素
DOI：
10.1007/s00441-020-03339-x
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发表时间：
2020
影响因子：
3.6
通讯作者：
Matsuda Seiji
中科院分区：
文献类型：
--
作者：
Shimokawa Tetsuya;Nabeka Hiroaki;Khan Sakirul Islam;Yamamiya Kimiko;Doihara Takuya;Kobayashi Naoto;Wakisaka Hiroyuki;Matsuda Seiji
鞘脂激活蛋白原（PSAP）有两种形式：前体和分泌形式。分泌形式具有神经营养、髓鞘营养和肌营养特性。前体形式是saposins A-D的前体蛋白。虽然PSAP在雄性生殖器官中的分布是众所周知的，但其在雌性生殖器官，特别是输卵管中的分布尚不清楚。输卵管的免疫印迹和免疫组化表明，输卵管组织中含有PSAP蛋白，并观察到PSAP显着增加，在发情后期阶段相比，发情间期-发情前期阶段的壶腹。为了鉴定PSAP在细胞中的运输，用抗PSAP的抗体与分拣蛋白、甘露糖6磷酸受体（M6 PR）或低密度脂蛋白受体相关蛋白1（LRP 1）组合进行双重免疫染色。PSAP和分拣蛋白双阳性反应观察到的核附近，以及在微绒毛上皮细胞的顶端部分，而这些反应只观察到纤毛上皮细胞的核附近。PSAP和M6 PR双阳性反应在微绒毛和纤毛上皮细胞的细胞核附近。PSAP和M6 PR双阳性反应也出现在微绒毛上皮细胞的顶端部分。PSAP和LRP 1双阳性反应在微绒毛和纤毛上皮细胞的质膜和顶端部分观察。免疫电子染色显示PSAP免疫反应性小泡与胞吐功能的微绒毛上皮细胞的顶端部分。这些研究结果表明，PSAP是存在于输卵管上皮细胞，并在怀孕期间在提供一个最佳的环境，配子和/或精子在壶腹中的关键作用。
Prosaposin (PSAP) has two forms: a precursor and a secreted form. The secreted form has neurotrophic, myelinotrophic, and myotrophic properties. The precursor form is a precursor protein of saposins A–D. Although the distribution of PSAP in male reproductive organs is well known, its distribution in female reproductive organs, especially in the oviduct, is unclear. Immunoblots and immunohistochemistry of oviducts showed that oviductal tissues contain PSAP proteins, and a significant increase in PSAP was observed in the estrus-metestrus phase compared to the diestrus-proestrus phase in the ampulla. To identify PSAP trafficking in cells, double-immunostaining was performed with antibodies against PSAP in combination with sortilin, mannose 6 phosphate receptor (M6PR), or low-density lipoprotein receptor-related protein 1 (LRP1). PSAP and sortilin double-positive reactions were observed near the nuclei, as well as in the apical portion of microvillous epithelial cells, whereas these reactions were only observed near the nuclei of ciliated epithelial cells. PSAP and M6PR double-positive reactions were observed near the nuclei of microvillous and ciliated epithelial cells. PSAP and M6PR double-positive reactions were also observed in the apical portion of microvillous epithelial cells. PSAP and LRP1 double-positive reactions were observed in the plasma membrane and apical portion of both microvillous and ciliated epithelial cells. Immunoelectron staining revealed PSAP immunoreactive small vesicles with exocytotic features at the apical portion of microvillous epithelial cells. These findings suggest that PSAP is present in the oviductal epithelium and has a pivotal role during pregnancy in providing an optimal environment for gametes and/or sperm in the ampulla.