Evaluation of a novel vesicular stomatitis virus pseudotype-based assay for detection of neutralizing antibody responses to SARS-CoV
Evaluation of a novel vesicular stomatitis virus pseudotype-based assay for detection of neutralizing antibody responses to SARS-CoV
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DOI：
10.1002/jmv.20732
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发表时间：
2006-12-01
影响因子：
12.7
通讯作者：
Morikawa, Shigeru
中科院分区：
文献类型：
--
作者：
Fukushi, Shuetsu;Mizutani, Tetsuya;Morikawa, Shigeru
严重急性呼吸综合征（SARS）冠状病毒（SARS-CoV）是SARS的病原体。SARS-CoV的S蛋白是感染患者中和抗体（Nabs）的主要靶点。我们开发了一种使用携带SARS-CoV-S蛋白的重组水泡性口炎病毒（VSV）（VSV-SARS-St 19）的中和试验。2003年SARS流行期间，从河内法国医院的22名医护人员中收集了56份血清样本，并与使用传染性SARS-CoV的传统中和试验进行了评价和比较。VSV-SARS-St 19假型中和试验结果与感染性SARS-CoV中和试验结果具有良好的相关性。新开发的中和试验对血清样品中的低抗体滴度更敏感。因此，VSV-SARS-St 19是检测SARS-CoV Nab的有用工具。
Severe acute respiratory syndrome (SARS)-coronavirus (SARS-CoV) is the causative agent of SARS. The S protein of SARS-CoV is a major target for neutralizing antibodies (Nabs) in infected patients. We developed a neutralization assay using a recombinant vesicular stomatitis virus (VSV) bearing SARS-CoV-S protein (VSV-SARS-St19). A total of 56 serum samples collected from 22 healthcare workers in the Hanoi French Hospital during the SARS epidemic in 2003 were evaluated and compared to the conventional neutralizing assay using infectious SARS-CoV. The results of the neutralization assay using VSV-SARS-St19 pseudotype showed good correlations with those using infectious SARS-CoV. The newly developed neutralization assay was more sensitive to low antibody titers in serum samples. Thus, the VSV-SARS-St19 is a useful tool for detecting Nabs against SARS-CoV.