The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules.
The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules.
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DOI：
10.1186/1471-2180-6-52
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发表时间：
2006-06-12
期刊：
影响因子：
4.2
通讯作者：
Stauffer GV
中科院分区：
文献类型：
--
作者：
McArthur SD;Pulvermacher SC;Stauffer GV
近年来，人们已经清楚地看到，小的非编码RNA在细菌毒力和细菌应激反应中起着调节元件的作用。我们测试了鼠疫杆菌中存在的小的非编码GcvB RNAs，作为这种生物中基因表达的可能调节因子。在本研究中，我们报道了鼠疫耶尔森菌KIM6gcvB基因编码两个小RNA。GcvB的转录被GCVA蛋白激活，被GcvR蛋白抑制。GcvB编码的RNA是抑制鼠疫杆菌DPPA基因所必需的，编码二肽运输系统的周质结合蛋白成分，表明GcvB RNAs具有调节活性。鼠疫耶尔森菌KIM6染色体gcvB基因的缺失会导致生物体世代时间的缩短和菌落形态的改变。本研究的结果表明，鼠疫杆菌gcvB基因编码两个小的非编码调控RNA，抑制DPPA的表达。GcvB的缺失是多效性的，这表明除了DPPA外，sRNAs可能还参与控制基因。
In recent years it has become clear that small non-coding RNAs function as regulatory elements in bacterial virulence and bacterial stress responses. We tested for the presence of the small non-coding GcvB RNAs in Y. pestis as possible regulators of gene expression in this organism. In this study, we report that the Yersinia pestis KIM6 gcvB gene encodes two small RNAs. Transcription of gcvB is activated by the GcvA protein and repressed by the GcvR protein. The gcvB-encoded RNAs are required for repression of the Y. pestis dppA gene, encoding the periplasmic-binding protein component of the dipeptide transport system, showing that the GcvB RNAs have regulatory activity. A deletion of the gcvB gene from the Y. pestis KIM6 chromosome results in a decrease in the generation time of the organism as well as a change in colony morphology. The results of this study indicate that the Y. pestis gcvB gene encodes two small non-coding regulatory RNAs that repress dppA expression. A gcvB deletion is pleiotropic, suggesting that the sRNAs are likely involved in controlling genes in addition to dppA.
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