Salmonella enterica Serovar Typhimurium Exploits Inflammation to Modify Swine Intestinal Microbiota.
Salmonella enterica Serovar Typhimurium Exploits Inflammation to Modify Swine Intestinal Microbiota.
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DOI：
10.3389/fcimb.2015.00106
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发表时间：
2015
影响因子：
5.7
通讯作者：
Magistrali CF
中科院分区：
文献类型：
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作者：
Drumo R;Pesciaroli M;Ruggeri J;Tarantino M;Chirullo B;Pistoia C;Petrucci P;Martinelli N;Moscati L;Manuali E;Pavone S;Picciolini M;Ammendola S;Gabai G;Battistoni A;Pezzotti G;Alborali GL;Napolioni V;Pasquali P;Magistrali CF
肠炎沙门氏菌血清型鼠伤寒沙门氏菌是一种重要的人畜共患胃肠道病原体，是世界范围内食源性疾病的主要病原体。它是一种成功的肠道病原体，因为它已经发展出了毒力策略，使其能够在高度炎症的肠道环境中生存，利用炎症来克服肠道微生物群提供的定植抗性。在这项研究中，我们使用具有完整微生物群的仔猪作为沙门氏菌病的模型，这些仔猪自然会发生肠胃炎。我们比较了野生型和减毒型鼠伤寒沙门氏菌对肠道微生物群的影响，以评估这些修饰是否与菌株的毒力相关。本研究表明，沙门氏菌以毒力依赖的方式改变菌群。我们发现野生型鼠伤寒沙门氏菌诱导炎症并减少通常参与提供病原体屏障的特定保护微生物群物种（产生scfa的细菌）。这两种作用都可能削弱定植抗性，增加宿主对野生型鼠伤寒沙门氏菌定植的易感性。相比之下，减毒的鼠伤寒沙门氏菌，其特点是定植肠道的能力降低，炎症反应非常轻微，无法成功地维持与微生物群的竞争。
Salmonella enterica serovar Typhimurium is an important zoonotic gastrointestinal pathogen responsible for foodborne disease worldwide. It is a successful enteric pathogen because it has developed virulence strategies allowing it to survive in a highly inflamed intestinal environment exploiting inflammation to overcome colonization resistance provided by intestinal microbiota. In this study, we used piglets featuring an intact microbiota, which naturally develop gastroenteritis, as model for salmonellosis. We compared the effects on the intestinal microbiota induced by a wild type and an attenuated S. Typhimurium in order to evaluate whether the modifications are correlated with the virulence of the strain. This study showed that Salmonella alters microbiota in a virulence-dependent manner. We found that the wild type S. Typhimurium induced inflammation and a reduction of specific protecting microbiota species (SCFA-producing bacteria) normally involved in providing a barrier against pathogens. Both these effects could contribute to impair colonization resistance, increasing the host susceptibility to wild type S. Typhimurium colonization. In contrast, the attenuated S. Typhimurium, which is characterized by a reduced ability to colonize the intestine, and by a very mild inflammatory response, was unable to successfully sustain competition with the microbiota.