Structural glycoprotein LmAbd-9 is required for the formation of the endocuticle during locust molting
Structural glycoprotein LmAbd-9 is required for the formation of the endocuticle during locust molting
复制标题
结构糖蛋白 LmAbd-9 是蝗虫蜕皮过程中内表皮形成所必需的
DOI：
10.1016/j.ijbiomac.2018.11.279
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发表时间：
2019
影响因子：
8.2
通讯作者：
Zhang Jianzhen
中科院分区：
文献类型：
--
作者：
Zhao Xiaoming;Jia Pan;Zhang Jing;Yang Yang;Liu Weimin;Zhang Jianzhen
昆虫角质层是由嵌入蛋白质基质中的几丁质丝组成的复合材料，主要由结构角质层蛋白组成。本研究基于飞蝗转录组对内皮结构糖蛋白基因LmAbd-9进行了表征。LmAbd-9编码具有几丁质结合域4的糖蛋白，属于CPR家族的RR-1亚类，具有两个可能发生糖基化修饰的潜在O-连接糖基化位点（S115和T137）。LmAbd-9在飞蝗中高表达。种皮并在蜕皮过程中表现出周期性表达。五龄若虫注射20-羟基蜕皮激素（20E）6、12和24 h后，LmAbd-9的表达水平显着下调；而在五龄若虫第2天，双链RNA介导的20E受体基因LmEcR和LmFTZ-F1β的RNA干扰48 h后，LmAbd-9的表达水平显着上调。五龄若虫注射dsLmAbd-9后第2天即可正常蜕皮为成虫，且无宏观表型表现；然而，成虫的角质层更薄，并且内表皮层明显少于对照。因此，受20E信号通路负调控的LmAbd-9参与了L中内表皮的形成。迁徙。
Insect cuticle is a composite made of chitin filaments embedded in a proteinaceous matrix, consisting mainly of structural cuticular proteins. In the present study, an endocuticle structural glycoprotein gene,LmAbd-9, was characterized based on theLocusta migratoriatranscriptome.LmAbd-9encodes a glycoprotein with a chitin binding domain 4, belonging to RR-1 subclass of the CPR family, which has two potential O-linked glycosylation sites (S115 and T137) at which glycosylation modification may occur.LmAbd-9was highly expressed in the integument and showed periodic expression during molting. The expression levels ofLmAbd-9were significantly down-regulated after injection with 20-hydroxyecdysone (20E) for 6, 12, and 24 h, whereas it was upregulated after double-stranded RNA-mediated RNA interference of the 20E receptor geneLmEcRandLmFTZ-F1betaat day 2 of fifth instar nymphs for 48 h. After injection of dsLmAbd-9on day 2 of fifth instar nymphs, the insects could normally molt to adults and showed no macroscopic phenotype; however, the cuticle of the adults was thinner, and there were significantly fewer endocuticular lamellae than in the control. Thus, LmAbd-9 that negatively regulated by the 20E signaling pathway was involved in the formation of the endocuticle inL. migratoria.