Long-term persistent infection of macaque monkeys with the simian immunodeficiency virus.
Long-term persistent infection of macaque monkeys with the simian immunodeficiency virus.
复制标题
DOI：
10.1099/0022-1317-68-12-3183
复制
发表时间：
1987-12
期刊：
影响因子：
--
通讯作者：
M. Daniel;N. Letvin;P. Sehgal;G. Hunsmann;D. Schmidt;N. King;R. Desrosiers
中科院分区：
文献类型：
--
作者：
M. Daniel;N. Letvin;P. Sehgal;G. Hunsmann;D. Schmidt;N. King;R. Desrosiers
通过静脉接种猴免疫缺陷病毒（SIV），实验性感染6至18个月龄的幼年恒河猴，SIV是与人类获得性免疫缺陷综合征（AIDS）病毒HIV相关的猴嗜T细胞逆转录病毒。用于接种的SIV在白细胞介素2存在下在正常人外周血淋巴细胞中或在人肿瘤细胞系HUT-78中生长。其中8只猕猴在接种后129至352天死亡，其临床和病理结果与人类艾滋病相似。然而，其他8只动物持续感染了很长一段时间;这8只猕猴在接种后537天和820天仍然活着，尽管存在持续的淋巴结病，我们仍然能够分离SIV。这些猴子在感染后存活的能力与它们对SIV的抗体反应强度直接相关。还使用约100个组织培养感染剂量的HUT-78生长的SIV在猕猴中建立感染。病毒接种物剂量与抗体应答强度或临床结局之间无相关性。这些结果表明，猕猴的SIV感染不仅可用于研究急性艾滋病，而且可用于模拟HIV携带者的长期持续感染。
Juvenile rhesus macaques 6 to 18 months of age were experimentally infected by intravenous inoculation with the simian immunodeficiency virus (SIV), the T cell-tropic retrovirus of monkeys related to the human acquired immunodeficiency syndrome (AIDS) virus HIV. The SIV used for inoculation was grown either in normal human peripheral blood lymphocytes in the presence of interleukin 2 or in the human tumour cell line HUT-78. Eight of the macaques died 129 to 352 days post-inoculation with a variety of clinical and pathological findings paralleling those of AIDS in humans. However eight other animals became persistently infected for prolonged periods; these eight macaques remained alive at 537 and 820 days post-inoculation despite persistent lymphadenopathy and our continued ability to isolate SIV. The ability of these monkeys to survive infection correlated directly with the strength of their antibody response to SIV. Infection was also established in macaques using approximately 100 tissue culture infectious doses of HUT-78-grown SIV. There was no correlation between the dose of virus inoculum and either the strength of the antibody response or clinical outcome. These results demonstrate that SIV infection of macaques can be used not only to study acute AIDS but also to mimic the long-term persistent infection seen in carriers of HIV.