RCA combined nanoparticle-based optical detection technique for protein microarray: a novel approach
RCA combined nanoparticle-based optical detection technique for protein microarray: a novel approach
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DOI：
10.1016/j.bios.2003.10.015
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发表时间：
2004-07-30
影响因子：
12.6
通讯作者：
Huang, YY
中科院分区：
文献类型：
--
作者：
Hsu, HY;Huang, YY
开发一种性能优良的生物传感器是许多研究小组的主要焦点。最近，分子分析微型化的重大突破已经产生了DNA和蛋白质微阵列。本课题组的目的是利用表面等离子体共振（SPR）技术建立一种灵敏的蛋白质微阵列信号分析技术。这种用于特异性分子结合的新检测技术利用滚环扩增（RCA）后信号处理方法[Nat. Genet. 19（1998）225-232]和通过微结构化芯片表面上的纳米金颗粒标记的分子的光学可视化。通过RCA引物与检测抗体的共价键合，保证了分析物与扩增的RCA产物之间的连接在测定期间得以维持。实验结果表明，RCA具有显着提高的灵敏度相比，传统的方法。这种易于检测的信号与芯片技术的结合应该有可能成为一个成功的商业应用。(C)2004 Elsevier B. V.保留所有权利。
Developing a readily available biosensor with excellent performances is the main focus of many research groups. Recently, major breakthroughs in miniaturization of molecular analysis have produced DNA and protein microarrays. The aim of our group is to develop a sensitive technique for analyzing signals on protein microarray by applying the surface plasmon resonance (SPR) method. This new detection technique for specific molecular binding utilizes rolling circles amplification (RCA) post-signal processing method [Nat. Genet. 19 (1998) 225-232] and optical visualization by nanogold particle-labeled molecules on a micro-structured chip surface. By covalent bonding of the RCA primer to the detection antibody guarantees that the linkage between the analyte and the amplified RCA product is maintained during the assay. Experimental results show that RCA has significantly enhanced sensitivity compared to conventional methods. This combination of an easily detectable signal with chip technology should have the potential to become a successful commercial application. (C) 2004 Elsevier B.V. All rights reserved.