Modification of Plant Height via RNAi Suppression of MdGA20-ox Gene Expression in Apple
Modification of Plant Height via RNAi Suppression of MdGA20-ox Gene Expression in Apple
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DOI：
10.21273/jashs.141.3.242
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发表时间：
2016-05
影响因子：
1.9
通讯作者：
K. Zhao;Feng Zhang;Yi Yang;Yue Ma;Yuexue Liu;He Li;H. Dai;Zhihong Zhang
中科院分区：
文献类型：
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作者：
K. Zhao;Feng Zhang;Yi Yang;Yue Ma;Yuexue Liu;He Li;H. Dai;Zhihong Zhang
一个束缚。赤霉素20-氧化酶（GA20-ox）是赤霉素（GA）生物合成的关键酶。为了研究MdGA 20-ox基因在植物生长发育中的作用，我们以苹果（Malus arctica cv. Hanfu）植物的RNA干扰。在温室中生长20周后，在转基因品系和非转基因对照之间观察到显著的表型差异。与非转基因对照相比，MdGA20-ox基因表达的抑制导致了较低的株高、较短的节间长度和较高的节数。转基因植株中MdGA 20-ox基因的表达明显受到抑制，转基因植株中活性GA含量低于非转基因对照。这些结果表明，MdGA20-ox基因在营养生长中起重要作用，因此，通过沉默MdGA20-ox基因来培育矮化或紧凑型接穗苹果品种是可能的。
A BSTRACT . GA20-oxidase (GA20-ox) is a key enzyme involved in the biosynthesis of gibberellic acid (GA). To investigate its role in plant growth and development, we suppressed MdGA20-ox gene expression in apple ( Malus domestica cv. Hanfu) plants by RNA interference (RNAi). After 20 weeks of growth in the greenhouse, significant phenotype differences were observed between transgenic lines and the nontransgenic control. Suppression of MdGA20-ox gene expression resulted in lower plant height, shorter internode length, and higher number of nodes compared with the nontransgenic control. The expression of MdGA20-ox in transgenic plants was significantly suppressed, and the active GA content in transgenic lines was lower than that in the nontransgenic control. These results demonstrated that the MdGA20-ox gene plays an important role in vegetative growth, and therefore it is possible to develop dwarfed or compact scion apple cultivars by MdGA20-ox gene silencing.