Repair during multifraction exposures: spheroids versus monolayers.
Repair during multifraction exposures: spheroids versus monolayers.
复制标题
多次暴露期间的修复：球体与单层。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
R. Durand
中科院分区：
文献类型：
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作者：
R. Durand
许多类型的哺乳动物细胞，当作为多细胞球体在培养物中生长时，显示出增强的积累和修复亚致死辐射损伤的能力，这被称为“接触效应”。由于这种效应有可能显着修改的多组分辐射反应的细胞在V79球体相对于单层培养的细胞，我们研究了方案范围从1到100个部分。选择接近1戈伊h-1的有效剂量率，以抑制细胞进展，从而简化结果分析。正如预期的那样，在两个系统中，每级分的较大剂量比每级分的较低剂量产生更多的净细胞杀伤。此外，在所有方案中观察到较少的球体细胞杀伤，这与其更大的修复潜力雅阁。然而，当数据表示为等效应曲线时，球形和单层曲线随着组分数量的增加而收敛。因此，对于每部分的超低剂量，将预测非常相似的固有灵敏度和修复能力。然而，用于定义0.2至1戈伊剂量辐射后存活率的高精度技术仍然能够证明生长为球状体的细胞的存活优势。
Many types of mammalian cells, when grown in culture as multicell spheroids, display an increased ability to accumulate and repair sublethal radiation damage which has been called the "contact effect". Since this effect has the potential to markedly modify the multifraction radiation response of cells in V79 spheroids relative to cells in monolayer cultures, we investigated regimens ranging from 1 to 100 fractions. Effective dose rates were chosen near 1 Gy h-1 to inhibit cell progression and thus simplify analysis of the results. As expected, larger doses per fraction produced more net cell killing in both systems than lower doses per fraction. Additionally, less killing of spheroid cells was observed in all regimens, in accord with their greater potential for repair. However, when the data were expressed as isoeffect curves, the spheroid and monolayer curves converged as the number of fractions increased. Thus, quite similar inherent sensitivity and repair capabilities would be predicted for ultra-low doses per fraction. High precision techniques for defining survival after doses of radiation from 0.2 to 1 Gy were, however, still able to demonstrate a survival advantage for cells grown as spheroids.