Bacterial alkaline phosphatase clonal variation in some Escherichia coli K-12 phoR mutant strains.
Bacterial alkaline phosphatase clonal variation in some Escherichia coli K-12 phoR mutant strains.
复制标题
一些大肠杆菌 K-12 phoR 突变株中的细菌碱性磷酸酶克隆变异。
DOI：
10.1128/jb.168.3.1366-1371.1986
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发表时间：
1986
影响因子：
3.2
通讯作者：
Wanner,BL
中科院分区：
文献类型：
--
作者：
Wanner,BL
几个phoR等位基因（phoR 19、phoR 20、phoR 68、phoR 69、phoR 70和phoR 78）导致细菌碱性磷酸酶（BAP）组成型表型或可变行为，具体取决于受试菌株。而大肠杆菌K10，MC 1000，和XPh 4 phoR突变体的组成，AB 1157，BD 792，MC 4100，和W3110 phoR突变体显示亚稳态字符。对于后者菌株，组成型突变体定期分离BAP阴性克隆，其再次以高频率产生组成型变体。事实上，在BAP-可变phoR菌株中观察到的变异模式在表型上类似于鼠伤寒沙门氏菌中H1/H2鞭毛抗原类型的阶段变异和λ噬菌体中免疫和敏感状态之间的分子开关。亚稳态行为不是BAP组成型突变体的一般特性，因为几个磷酸盐特异性转运-phoU突变导致组成型（稳定）表型，无论测试的菌株如何。但在phoR磷酸盐特异性转运-phoU突变体中，亚稳性状是上位性的（显性的），并且这种双突变体在BAP可变菌株中表现出克隆变异。
Several phoR alleles (phoR19, phoR20, phoR68, phoR69, phoR70, and phoR78) led to either a bacterial alkaline phosphatase (BAP)-constitutive phenotype or a variable behavior, depending upon the strain tested. Whereas Escherichia coli K10, MC1000, and XPh4 phoR mutants were constitutive, AB1157, BD792, MC4100, and W3110 phoR mutants displayed the metastable character. For the latter strains, constitutive mutants regularly segregated BAP-negative clones which yielded constitutive variants again at a high frequency. Indeed, the pattern of variation observed in BAP-variable phoR strains is phenotypically analogous to phase variation of the H1/H2 flagellum antigen type in Salmonella typhimurium and the molecular switch between the immune and sensitive states in bacteriophage lambda. The metastable behavior was not a general property of BAP-constitutive mutants, since several phosphate-specific transport-phoU mutations led to a constitutive (stable) phenotype regardless of the strain tested. But in phoR phosphate-specific transport-phoU mutants, the metastable character was epistatic (dominant), and such double mutants showed clonal variation in BAP-variable strains.