Plasmin-treated low density lipoproteins: polypeptide analyses and metabolism by cultured smooth muscle cells.
Plasmin-treated low density lipoproteins: polypeptide analyses and metabolism by cultured smooth muscle cells.
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纤溶酶处理的低密度脂蛋白：培养的平滑肌细胞的多肽分析和代谢。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
D. R. van der Westhuyzen
中科院分区：
文献类型：
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作者：
G. Coetzee;W. Gevers;D. R. van der Westhuyzen
纤溶酶由尿激酶与纤溶酶原相互作用产生，可降解人低密度脂蛋白的脱辅基蛋白 B 部分，在只有一小部分（小于 3%）蛋白质水解为三氯乙酸可溶产物的条件下产生独特的高分子量中间体。通过 SDS-聚丙烯酰胺电泳估计这些中间体的分子量在 60 000 至 200 000 之间。胰蛋白酶处理产生的片段与用纤溶酶获得的片段大小相似。当将酶处理的低密度脂蛋白添加到培养的牛主动脉平滑肌细胞中时，受体结合以及内化和降解速率与天然低密度脂蛋白的情况下获得的结果没有不同。
Plasmin, generated by the interaction of urokinase with plasminogen, degraded the apoprotein B moiety of human low density lipoprotein to yield distinct high moleculr weight intermediates under conditions where only a small fraction (less than 3%) of the protein was hydrolyzed to trichloroacetic acid-soluble products. The molecular weights of these intermediates were between 60 000 and 200 000 as estimated by SDS-polyacrylamide electrophoresis. Trypsin treatment yielded fragments of similar size to those obtained with plasmin. When enzyme-treated low density lipoproteins were added to bovine aortic smooth muscle cells in culture, the receptor-binding, and rates of internalization and degradation were no different from those obtained in the case of native low density lipoproteins.