Diabetes mellitus is associated with increased intramyocellular triglyceride, but not diglyceride, content in obese humans
Diabetes mellitus is associated with increased intramyocellular triglyceride, but not diglyceride, content in obese humans
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DOI：
10.1016/j.metabol.2009.05.019
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发表时间：
2009-11-01
影响因子：
9.8
通讯作者：
Melidonis, Adreas
中科院分区：
文献类型：
--
作者：
Anastasiou, Costas A.;Kavouras, Stavros A.;Melidonis, Adreas
研究表明，肌细胞内甘油二酯可能与胰岛素抵抗有关，因此可能与糖尿病的病理生理学有关。我们的目的是研究糖尿病患者肌细胞内甘油二酯和甘油三酯水平，并探讨其与血糖控制的可能联系。该研究的参与者是30名肥胖受试者，根据糖尿病的存在分为非糖尿病性肥胖（n = 19）和糖尿病性肥胖（n = 11）。肌细胞内甘油三脂和甘油二脂是在禁食条件下从股外侧肌活检确定的。分别通过口服葡萄糖耐量试验和稳态模型评估血糖控制和胰岛素抵抗。与非糖尿病肥胖组相比，糖尿病肥胖组的肌细胞内甘油三酯水平更高（分别为66.67 +/- 23.75 vs 18.35 +/- 4.42 nmol.mg（-1）干组织; P <0.05）。研究组之间的甘油二酯水平无显著差异（分别为1.65 +/- 0.27 vs 1.94 +/- 0.65 nmol.mg（-1）干组织）。单不饱和脂肪酸是两组肌细胞内甘油三酯的主要成分，而甘油二酯主要含有饱和脂肪酸。肌细胞内甘油三酯水平与血糖控制（以葡萄糖曲线下面积表示）之间存在显著相关性（r = 0.417，P <0.05）。两类脂质的肌细胞内水平与胰岛素抵抗之间没有相关性。我们的数据支持血糖控制和肌内甘油三酯之间的关系，而不是甘油二酯。脂肪酸向酯化的总流量可能是糖尿病病理生理学中一个重要得多的因素。(C)2009 Elsevier Inc. All rights reserved.
It has been suggested that intramyocellular diglycerides may be associated with insulin resistance and thus may be linked to the pathophysiology of diabetes. We aimed to investigate intramyocellular diglyceride as well as triglyceride levels in diabetic subjects and to explore a possible association with glycemic control. The participants of the study were 30 obese subjects stratified according to the presence of diabetes into nondiabetic obese (n = 19) and diabetic obese (n = 11). Intramyocellular triglycerides and diglycerides were determined ill biopsies from the vastus lateralis muscle under fasting conditions. Glycemic control and insulin resistance were assessed by an oral glucose tolerance test and the homeostatic model, respectively. Higher levels of intramyocellular triglycerides were observed in the diabetic obese group compared with the nondiabetic obese group (66.67 +/- 23.75 vs 18.35 +/- 4.42 nmol.mg(-1) dry tissue, respectively; P < .05). Diglyceride levels were not significantly different between the study groups (1.65 +/- 0.27 vs 1.94 +/- 0.65 nmol.mg(-1) dry tissue, respectively). Monounsaturated fatty acids represented the major constituent of intramyocellular triglycerides in both groups, whereas diglycerides contained mainly saturated fatty acids. A significant correlation was found between intramyocellular levels of triglycerides, but not diglycerides, and glycemic control, expressed as the area under the glucose curve (r = 0.417, P < .05). No correlations were found between intramyocellular levels of both lipid classes and insulin resistance. Our data support a relationship between glycemic control and intramyocellular triglycerides, but not diglycerides. The total flux of fatty acids toward esterification may be a much more important factor in the pathophysiology of diabetes. (C) 2009 Elsevier Inc. All rights reserved.