Isolation and maintenance of in vitro cell cultures from the ctenophore Mnemiopsis leidyi
Isolation and maintenance of in vitro cell cultures from the ctenophore Mnemiopsis leidyi
复制标题
栉水母 Mnemiopsis leidyi 体外细胞培养物的分离和维持
DOI：
10.1007/978-1-0716-2172-1_18
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发表时间：
2022
影响因子：
--
通讯作者：
Browne W.E.
中科院分区：
文献类型：
--
作者：
Dieter A.C.;Vandepas L.E.;Browne W.E.
分离、监测和检查感兴趣的特定细胞的能力使得能够进行靶向实验操作，否则在整个生物体的背景下难以进行和解释。在体外原代细胞培养物来自栉水母，因此作为一个重要的工具，了解复杂的细胞和分子的相互作用，发生在不同的栉水母细胞类型之内和之间。在这里，我们描述了可靠地产生和维持来自叶状栉水母Mnemiopsis leidyi，可用于各种各样的实验应用的原代细胞培养物的方法。
The ability to isolate, monitor, and examine specific cells of interest enables targeted experimental manipulations that would otherwise be difficult to perform and interpret in the context of the whole organism. In vitro primary cell cultures derived from ctenophores thus serve as an important tool for understanding complex cellular and molecular interactions that take place both within and between various ctenophore cell types. Here we describe methods for reliably generating and maintaining primary cell cultures derived from the lobate ctenophore Mnemiopsis leidyi that can be used for a wide variety of experimental applications.