COMMON CONDITIONS FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC MICRODETERMINATION OF ALDOSES, HEXOSAMINES, AND SIALIC ACIDS IN GLYCOPROTEINS
COMMON CONDITIONS FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC MICRODETERMINATION OF ALDOSES, HEXOSAMINES, AND SIALIC ACIDS IN GLYCOPROTEINS
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DOI：
10.1016/0003-2697(84)90533-5
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发表时间：
1984-01-01
影响因子：
2.9
通讯作者：
SUZUKI, S
中科院分区：
文献类型：
--
作者：
HONDA, S;SUZUKI, S
所有已知存在于动物和植物糖蛋白中的醛糖均在高度交联的阳离子交换树脂（Shodex DC-613，H+ 形式）柱上以分配模式分离，并在用 2-氰基乙酰胺进行柱后标记后通过光度检测进行灵敏监测。观察到样品量在 0.1-80 nmol 范围内呈线性且具有高重现性。己糖胺可以在与 .apprx 相同的条件下测定为其 N-乙酰基衍生物。线性范围提高 1 个数量级。唾液酸可以估计为由 N-乙酰神经氨酸丙酮酸裂解酶作用产生的 N-酰基己糖胺。通过分析一些糖蛋白制剂的单糖成分，证明了这些常见条件的有用性。
All aldoses known to be present in animal and plant glycoproteins were separated in partition mode on a column of a highly crosslinked cation-exchange resin (Shodex DC-613, H+ form) and sensitively monitored by photometric detection after postcolumn labeling with 2-cyanoacetamide. Linearity for the sample amount ranging from 0.1-80 nmol was observed with high reproducibility. Hexosamines could be determined as their N-acetyl derivatives under the identical conditions with .apprx. 1 order of magnitude higher range of linearity. Sialic acid could be estimated as N-acylhexosamines produced by the action of N-acetylneuraminate pyruvate-lyase. The usefulness of these common conditions was demonstrated by analyzing component monosaccharides of some glycoprotein preparations.