Effects of insemination-ovulation interval on fertilization rates and embryo characteristics in dairy cattle
Effects of insemination-ovulation interval on fertilization rates and embryo characteristics in dairy cattle
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DOI：
10.1016/j.theriogenology.2006.07.005
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发表时间：
2006-12-01
期刊：
影响因子：
2.8
通讯作者：
Kemp, B.
中科院分区：
文献类型：
--
作者：
Roelofs, J. B.;Graat, E. A. M.;Kemp, B.
本研究的目的是检查授精和排卵之间的间隔对奶牛受精和胚胎特征（质量评分为好，一般，差和退化;形态;细胞周期数和辅助精子数）的影响。通过超声检查（每4小时）评估排卵时间。母牛在排卵前36天至排卵后12小时人工授精一次。排卵后7 d共回收122枚卵母细胞/胚胎。诱导排卵间隔（12小时间隔）影响受精和好胚胎的百分比。与排卵后AI（56%）相比，排卵前AI在36-24和24-12之间进行时的受精率更高（85%和82%）。排卵前24至12小时的AI导致良好胚胎的百分比（68%）高于排卵后的AI（6%）。当校正胚胎质量时，排卵间隔对辅助精子细胞数和细胞周期数没有影响。这项研究表明，具有高受精概率的排卵间隔相当长（从排卵前的36到12 It）。然而，这种受精卵母细胞很有可能发育成一个好胚胎的受精-排卵间隔较短（排卵前24-12小时）。(c)2006年爱思唯尔公司All rights reserved.
The objective of this study was to examine effects of the interval between insemination and ovulation on fertilization and embryo characteristics (quality scored as good, fair, poor and degenerate; morphology; number of cell cycles and accessory sperm number) in dairy cattle. Time of ovulation was assessed by ultrasonography (every 4 h). Cows were artificially inseminated once between 36 It before ovulation and 12 h after ovulation. In total 122 oocytes/embryos were recovered 7 d after ovulation. Insemination-ovulation interval (12 h-intervals) affected fertilization and the percentages of good embryos. Fertilization rates were higher when AI was performed between 36-24 and 24-12 It before ovulation (85% and 82%) compared to AI after ovulation (56%). AI between 24 and 12 h before ovulation resulted in higher percentages of good embryos (68%) compared to AI after ovulation (6%). Insemination-ovulation interval had no effect on number of accessory sperm cells and number of cell cycles when corrected for embryo quality. This study showed that the insemination-ovulation interval with a high probability of fertilization is quite long (from 36 to 12 It before ovulation). However, the insemination-ovulation interval in which this fertilized oocyte has a high probability of developing into a good embryo is shorter (24-12 It before ovulation). (c) 2006 Elsevier Inc. All rights reserved.