The geometry of domain combination in proteins
The geometry of domain combination in proteins
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DOI：
10.1006/jmbi.2001.5288
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发表时间：
2002-01-25
影响因子：
5.6
通讯作者：
Chothia, C
中科院分区：
文献类型：
--
作者：
Bashton, M;Chothia, C
基因组中的大多数蛋白质是两个或多个结构域重组的结果。已经发现，如果蛋白质由来自超家族A和B的结构域的组合形成，则结构域可以以连续顺序AB或BA出现，但仅在约2%的情况下它们以两种连续顺序出现。已知结构的经典Rossmann结构域与来自七个不同超家族的催化结构域相结合。此外，有八种情况下，结构与AB和BA结构域的组合是已知的。对于这两组结构，我们分析了：（i）域的相对取向;（ii）域连接的类型;（iii）域间链接的结构;和（iv）域功能。这一分析的结果表明，在大多数情况下，结构域的顺序是保守的，因为重组的结构域只发生了一次在进化过程中。当域连接较短时，功能性原因变得很重要。在已知的八种情况中，有七种情况下，域以AB和BA序列顺序组合，它们具有不同的几何关系，赋予它们不同的功能特性。(C)北京大学出版社.
Most proteins in genomes are the result of the recombination of two or more domains. It has been found that if proteins are formed by a combination of domains from superfamilies A and B, then the domains may occur in the sequential order AB or BA but only in about 2 % of cases do they occur in both sequential orders. The classical Rossmann domains of known structure are combined with catalytic domains from seven different superfamilies. In addition, there are eight cases where structures with both AB and BA domain combinations are known. For these two sets of structures, we analysed: (i) the relative orientation of the domains; (ii) the type of domain connection; (iii) the structure of the interdomain links; and (iv) domain function. The results of this analysis indicate that in most cases domain order is conserved because recombination of the domains has only occurred once during the course of evolution. Functional reasons become important when the domain connections are short. In seven out of the eight known cases where domains are combined in the AB and BA sequential orders they have different geometrical relationships that give them different functional properties. (C) 2002 Academic Press.