Visualization of Aspergillus fumigatus biofilms with Scanning Electron Microscopy and Variable Pressure-Scanning Electron Microscopy: A comparison of processing techniques
Visualization of Aspergillus fumigatus biofilms with Scanning Electron Microscopy and Variable Pressure-Scanning Electron Microscopy: A comparison of processing techniques
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DOI：
10.1016/j.mimet.2016.11.002
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发表时间：
2017-01-01
影响因子：
2.2
通讯作者：
Cegelski, Lynette
中科院分区：
文献类型：
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作者：
Joubert, Lydia-Marie;Ferreira, Jose A. G.;Cegelski, Lynette
烟曲霉生物膜由菌丝和细胞外基质组成的三维网状结构组成。它们参与免疫受损个体的感染，特别是那些患有囊性纤维化的个体。这些结构与感染的持续性、对宿主免疫的抗性和抗微生物剂抗性相关。对结构和功能的透彻理解在治疗药物的设计中是必不可少的。优化的处理参数，包括醛固定，重金属对比，干燥技术和离子液体处理，进行了超微结构的方法来了解细胞和细胞外生物膜成分。应用常规和变压扫描电子显微镜分析附着在塑料上的生物膜的结构，并形成在气液界面。(C)© 2016 Elsevier B.V.版权所有。
Aspergillus fumigatus biofilms consist of a three-dimensional network of cellular hyphae and extracellular matrix. They are involved in infections of immune-compromised individuals, particularly those with cystic fibrosis. These structures are associated with persistence of infection, resistance to host immunity, and antimicrobial resistance. Thorough understanding of structure and function is imperative in the design of therapeutic drugs. Optimization of processing parameters, including aldehyde fixation, heavy metal contrasting, drying techniques and Ionic Liquid treatment, was undertaken for an ultrastructural approach to understand cellular and extracellular biofilm components. Conventional and Variable Pressure Scanning Electron Microscopy were applied to analyze the structure of biofilms attached to plastic and formed at an air-liquid interface. (C) 2016 Elsevier B.V. All rights reserved.