Quantitative serial sectioning analysis
Quantitative serial sectioning analysis
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DOI：
10.1046/j.1365-2818.2001.00832.x
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发表时间：
2001-03-01
期刊：
影响因子：
--
通讯作者：
Voorhees, PW
中科院分区：
文献类型：
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作者：
Alkemper, J;Voorhees, PW
一种连续切片的方法，它允许每小时取约20个切片，切片之间的间距在1-20 μ m的范围内。横截面的对齐是用线性可变差分Transformer完成的;因此，它与样品的微观结构无关，并且不依赖于植入样品中的标记。与新方法相关的对准误差以及节之间的倾斜和旋转误差被发现是可以忽略的。一旦所有的部分都被计算机捕获，就可以构建三维图像。该图像可以交互式查看和旋转，从而允许直接观察三维形状。它可以进一步用于确定大量的微观结构参数，包括那些不能从平面截面确定。通过对铸造标准铝合金试样微观结构的重建，说明了该技术。
A method for serial sectioning is presented that allows one to take about 20 sections per hour with spacings in the range 1-20 mum between sections. The alignment of the cross-sections is done with a linear variable differential transformer; it is thus independent of the microstructure of the sample and does not rely upon markers implanted in the sample. The alignment errors as well as tilts and rotation errors between sections associated with the new method are found to be negligible. Once all the sections are captured in a computer a three-dimensional image can be constructed. This image can be viewed interactively and rotated, thus allowing the direct observation of three-dimensional shapes. It can further be used to determine a vast array of microstructural parameters including those that cannot be determined from planar sections. The technique is illustrated through the reconstruction of the microstructure of a cast standard aluminium alloy specimen.