Attenuation of age-dependent oxidative damage to DNA and protein in brainstem of Tg Cu/Zn SOD mice
Attenuation of age-dependent oxidative damage to DNA and protein in brainstem of Tg Cu/Zn SOD mice
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DOI：
10.1016/s0197-4580(98)00067-0
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发表时间：
1998-07-01
影响因子：
4.2
通讯作者：
Sanchez-Ramos, J
中科院分区：
文献类型：
--
作者：
Cardozo-Pelaez, F;Song, S;Sanchez-Ramos, J
在正常和转基因（tg）小鼠过表达人铜/锌超氧化物歧化酶（h-SOD 1）的脑干和纹状体中的氧化DNA和蛋白质损伤的神经元依赖性积累进行了评估。在对照组和tg小鼠的3、12和18个月龄时测量了氧化性DNA损伤的标志物8-羟基-2 '-脱氧鸟苷（oxo（8）dG）。Cu/Zn SOD，但不MnSOD，从tg小鼠脑干和纹状体中的活性增加，在所有年龄组与对照组相比。在18个月时，对照小鼠的脑干中oxo 8dG水平增加了58%，纹状体中增加了21%。在转基因小鼠，脑干和纹状体oxo 8dG水平增加到一个较小的程度比相应的控制。18个月时，对照组脑干中的蛋白质氧化（羰基含量）增加了59%，但纹状体中没有，并且在tg小鼠中这种增加明显减弱。总之，随着年龄的增长，脑干中DNA和蛋白质的氧化损伤增加，纹状体中的氧化损伤程度较轻，而Cu/Zn SOD活性的增加改变了DNA和蛋白质损伤的程度。(C)1998年爱思唯尔科学公司
Age-dependent accumulation of oxidative DNA and protein damage in brainstem and striatum was assessed in normal and transgenic (tg) mice which overexpress human Cu/Zn superoxide dismutase (h-SOD1). A marker of oxidative DNA damage, 8-hydroxy-2'-deoxyguanosine (oxo(8)dG), was measured at 3, 12, and 18 months of age in control and tg mice. Cu/Zn SOD, but not MnSOD, activities in brainstems and striata from tg mice were increased compared to controls at all ages. At 18 months, oxo8dG levels were increased by 58% in brainstem and by 21% in striatum of control mice. In the tg mice, brainstem and striatal oxo8dG levels were increased to a lesser extent than in the corresponding controls. Protein oxidation (carbonyl content), was increased by 59% at 18 months in control brainstem, but not in striatum, and the increase was significantly attenuated in the tg mice. In summary, oxidative damage to DNA and protein increased with age in brainstem land to a lesser extent in striatum), and augmented Cu/Zn SOD activity modified the extent of DNA and protein damage. (C) 1998 Elsevier Science Inc.