Membrane antigen on Epstein--Barr virus-infected human B cells recognized by a monoclonal antibody.
Membrane antigen on Epstein--Barr virus-infected human B cells recognized by a monoclonal antibody.
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单克隆抗体识别感染 Epstein-Barr 病毒的人 B 细胞上的膜抗原。
DOI：
10.1073/pnas.79.8.2649
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发表时间：
1982
影响因子：
11.1
通讯作者：
Vaughan,JH
中科院分区：
文献类型：
--
作者：
Slovin,SF;Frisman,DM;Tsoukas,CD;Royston,I;Baird,SM;Wormsley,SB;Carson,DA;Vaughan,JH
本文描述了一种单克隆抗体（B532），其检测来自携带EB病毒（EBV）基因组的细胞系的大于或等于95%的B细胞上存在的膜抗原。表明B532与EBV相关的证据最初是通过使用不同细胞系底物的细胞结合放射性测定获得的。免疫荧光和细胞分选分析证实，抗原是目前在所有EBV感染的淋巴母细胞样B细胞系的高密度，但不是在EBV阴性B-，T-，髓样，或空细胞系。分离的正常外周血B和T淋巴细胞和单核细胞不能结合B532。该单克隆抗体不抑制体外EVB感染，也不阻断细胞毒性T淋巴细胞对EBV感染靶细胞的杀伤。通过免疫沉淀显示，B532识别的细胞表面抗原具有约45，000的分子量。
This paper describes a monoclonal antibody (B532) that detects a membrane antigen present on greater than or equal to 95% of the B cells from lines carrying the Epstein-Barr virus (EBV) genome. Evidence suggesting that B532 is EBV-related was originally obtained by using a cell-binding radioassay with different cell line substrates. Immunofluorescence and cell-sorter analysis confirmed that the antigen was present in high density on all EBV-infected lymphoblastoid B-cell lines, but not on EBV-negative B-, T-, myeloid, or null cell lines. Isolated normal peripheral blood B and T lymphocytes and monocytes failed to bind B532. The monoclonal antibody did not inhibit in vitro EVB infection nor did it block the killing of EBV-infected targets by cytotoxic T lymphocytes. The cell surface antigen recognized by B532 was shown by immunoprecipitation to have a molecular weight of approximately 45,000.