Nucleotide excision repair is reduced in oral epithelial tissues compared with skin.
Nucleotide excision repair is reduced in oral epithelial tissues compared with skin.
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DOI：
10.1111/j.1751-1097.2012.01163.x
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发表时间：
2012-07
影响因子：
3.3
通讯作者：
Godar D
中科院分区：
文献类型：
--
作者：
Mitchell D;Paniker L;Godar D
在过去十年中，用于诊断、治疗和美容的内部组织紫外线辐射（UVR）暴露急剧增加。紫外线照射内部组织的最大增加发生在化妆品行业，它与氧化剂一起用于牙齿美白，通常与室内晒黑一起使用。为了解决这些过程的潜在致癌风险，我们分析了与UVR特征突变相关的DNA光产物的形成和修复。放射免疫测定法用于定量从三种重建组织（EpiDerm™，EpiGingival™和EpiOral™）中纯化的DNA中环丁烷嘧啶二聚体和嘧啶（6-4）嘧啶光产物的诱导和修复。我们在紫外线照射后立即观察到所有组织的DNA损伤水平相当。相比之下，与表皮™相比，两种口腔组织的修复都明显减少。我们的数据表明，口腔组织暴露在紫外线下会导致DNA损伤的积累，增加患口腔癌和黑色素瘤的风险。由于NER是一种广谱防御除UVR外由多种物质引起的DNA损伤，我们的数据表明，在这种高度暴露的环境中，口腔组织中观察到的相对较低的NER效率可能会产生广泛的后果。
Ultraviolet radiation (UVR) exposure to internal tissues for diagnostic, therapeutic and cosmetic procedures has increased dramatically over the past decade. The greatest increase in UVR exposure of internal tissues occurs in the cosmetic industry where it is combined with oxidizing agents for teeth whitening, often in conjunction with indoor tanning. To address potential carcinogenic risks of these procedures, we analyzed the formation and repair of the DNA photoproducts associated with the signature mutations of UVR. Radioimmunoassay was used to quantify the induction and repair of cyclobutane pyrimidine dimers and pyrimidine(6-4)pyrimidone photoproducts in DNA purified from three reconstructed tissues, EpiDerm™, EpiGingival™, and EpiOral™ (MatTek Corp.). We observed comparable levels of DNA damage in all tissues immediately after UVR exposure. In contrast, repair was significantly reduced in both oral tissues compared to EpiDerm™. Our data suggest that UVR exposure of oral tissues can result in accumulation of DNA damage and increase the risk for carcinoma and melanoma of the mouth. Because NER is a broad-spectrum defense against DNA damage caused by a variety of agents in addition to UVR, our data suggest that the relatively low NER efficiency observed in oral tissues may have wide-ranging consequences in this highly exposed environment.
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影响因子：
11.2
作者：
Li, Jinyou;Wang, Qi-En;Wani, Altaf A.
通讯作者：
Wani, Altaf A.
影响因子：
6.6
作者：
Anidjar, M;Ettori, D;Avrillier, S
通讯作者：
Avrillier, S
影响因子：
1.4
作者：
McGee, Sasha;Mardirossian, Vartan;Feld, Michael S.
通讯作者：
Feld, Michael S.
DOI：
10.1016/1011-1344(95)07178-4
发表时间：
1995-12-01
影响因子：
5.4
作者：
Pradhan, A;Pal, P;Blanchard, L
通讯作者：
Blanchard, L
影响因子：
3.5
作者：
Alimova, Alexandra;Katz, A.;Alfano, R. R.
通讯作者：
Alfano, R. R.