Multiscale Vascular Enhancement Filter Applied to In Vivo Morphologic and Functional Photoacoustic Imaging of Rat Ocular Vasculature
Multiscale Vascular Enhancement Filter Applied to In Vivo Morphologic and Functional Photoacoustic Imaging of Rat Ocular Vasculature
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DOI：
10.1109/jphot.2019.2948955
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发表时间：
2019-10
影响因子：
2.4
通讯作者：
Huangxuan Zhao;Chengbo Liu;Ke Li;Ningbo Chen;Kunya Zhang;Lidai Wang;Riqiang Lin;Xiaojing Gong;Liang Song;Zhicheng Liu
中科院分区：
文献类型：
--
作者：
Huangxuan Zhao;Chengbo Liu;Ke Li;Ningbo Chen;Kunya Zhang;Lidai Wang;Riqiang Lin;Xiaojing Gong;Liang Song;Zhicheng Liu
光学分辨光声显微镜（Optical-resolution photoacoustic microscopy，OR-PAM）具有不需要外源性染料、成像分辨率高、可同时实现形态和功能成像等优点，可用于各种白化病和色素眼的活体成像。然而，为了准确地诊断OR-PAM图像中的眼科疾病，血管增强算法对于提取血管并正确地量化它们是必要的。为其他成像技术开发的血管增强算法不适合用于OR-PAM，因为图像形成的物理学存在根本差异。在这项研究中，一种新的血管增强算法称为光声成像血管增强滤波器（PAIVEF），它不仅增强血管，包括微血管信号，有效地抑制噪声信号，但也实现了高灵敏度和准确的血管增强在一个大的深度范围内和系统的焦深（DOF）。PAIVEF系统可同时显示大鼠眼前节血管的形态和功能三维图像，深度范围为0.6mm，是系统自由度的1.7倍。本研究为OR-PAM技术在眼科疾病研究中的应用奠定了基础。
Optical-resolution photoacoustic microscopy (OR-PAM) is used for in vivo imaging of a variety of albino and pigmented eyes taking advantages of requiring no exogenous dye, performing high-resolution imaging, and achieving morphologic and functional imaging at the same time. However, to accurately diagnose the ophthalmic disease in the OR-PAM images, vascular enhancement algorithms are necessary for extracting vessels and quantifying them correctly. Vascular enhancement algorithms developed for other imaging technologies, are not suitable to be used for OR-PAM, because of the underlying differences in the physics of the formation of images. In this study, a new vascular enhancement algorithm called photoacoustic imaging vasculature enhancement filter (PAIVEF) is proposed, which not only enhances vasculature including micro-vessels signals, suppresses noise signals effectively, but also achieves highly sensitive and accurate enhancement of the vasculature within a large depth range in and out of the system's depth of focus (DOF). Using the PAIVEF, the morphologic and functional 3D images of the whole rat's ocular anterior vasculature segment was displayed simultaneously for a depth range of ∼0.6 mm, which was ∼7 times of the system's DOF. This study paves the way for the application of OR-PAM technology in ophthalmic disease research.