A conserved Toll-like receptor-to-NF-κB signaling pathway in the endangered coral Orbicella faveolata
A conserved Toll-like receptor-to-NF-κB signaling pathway in the endangered coral Orbicella faveolata
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DOI：
10.1016/j.dci.2017.10.016
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发表时间：
2018-02-01
影响因子：
2.9
通讯作者：
Gilmore, Thomas D.
中科院分区：
文献类型：
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作者：
Williams, Leah M.;Fuess, Lauren E.;Gilmore, Thomas D.
在这里，我们的特点Toll样受体（TLR）到NF-κ B天然免疫途径的Orbicella faveolata（Of），这是一个生态上重要的，疾病易感，造礁珊瑚。与人TLR相比，Of-TLR的细胞内TIR结构域与TLR 4最相似，并且它可以在体外与人TLR 4衔接子MYD 88相互作用。治疗O.用脂多糖（哺乳动物TLR 4的配体）处理蜂窝组织，导致与NF-κ B途径动员一致的基因表达变化。生物化学和基于细胞的测定显示，Of-NB-κ B类似于哺乳动物非典型NF-κ B蛋白p100，因为C-末端截短导致Of-NF-κ B易位到细胞核并增加其DNA结合和转录激活活性。此外，人I κ B激酶（IKK）和Of-IKK在体外都可以磷酸化Of-NF-κ B中的保守残基，并在体内诱导Of-NF-κ B的C末端加工。这些结果是濒危珊瑚中TLR-到-NF-κ B信号蛋白的第一个特征，并表明这些珊瑚具有保守的先天免疫途径。(C)2017爱思唯尔有限公司版权所有
Herein, we characterize the Toll-like receptor (TLR)-to-NF-kappa B innate immune pathway of Orbicella faveolata (Of), which is an ecologically important, disease-susceptible, reef-building coral. As compared to human TLRs, the intracellular TIR domain of Of-TLR is most similar to TLR4, and it can interact in vitro with the human TLR4 adapter MYD88. Treatment of O. faveolata tissue with lipopolysaccharide, a ligand for mammalian TLR4, resulted in gene expression changes consistent with NF-kappa B pathway mobilization. Biochemical and cell-based assays revealed that Of-NB-kappa B resembles the mammalian non-canonical NF-kappa B protein p100 in that C-terminal truncation results in translocation of Of-NF-kappa B to the nucleus and increases its DNA-binding and transcriptional activation activities. Moreover, human I kappa B kinase (IKK) and Of-IKK can both phosphorylate conserved residues in Of-NF-kappa B in vitro and induce C-terminal processing of Of-NF-kappa B in vivo. These results are the first characterization of TLR-to-NF-kappa B signaling proteins in an endangered coral, and suggest that these corals have conserved innate immune pathways. (C) 2017 Elsevier Ltd. All rights reserved.