Increasing morphinan alkaloid production by over-expressing codeinone reductase in transgenic Papaver somniferum
Increasing morphinan alkaloid production by over-expressing codeinone reductase in transgenic Papaver somniferum
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DOI：
10.1111/j.1467-7652.2006.00212.x
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发表时间：
2007-01-01
影响因子：
13.8
通讯作者：
Fist, Anthony J.
中科院分区：
文献类型：
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作者：
Larkin, Philip J.;Miller, James A. C.;Fist, Anthony J.
只有罂粟属植物（罂粟）才能合成吗啡烷生物碱，罂粟的种植对吗啡、可待因和各种半合成镇痛剂的生产和供应仍然至关重要。罂粟转化可待因酮还原酶（PsCor1.1），吗啡合成的倒数第二步的组成型表达的cDNA。大多数转基因株系表现出显着增加，在重复的温室和田间试验超过4年的胶囊生物碱含量。吗啡烷生物碱含量在干重基础上的15%和30%之间，高于对照高产基因型和对照非转基因分离体。与非转基因对照相比，转基因叶片具有大约10倍的Cor转录水平。两个周期的最佳转基因线到精英高吗啡基因型的交叉导致吗啡和总生物碱相对于精英经常性的父母显着增加。在叶、根、花粉和种子中未观察到生物碱谱或量的显著变化。
Only plants of the Papaver genus (poppies) are able to synthesize morphinan alkaloids, and cultivation of P. somniferum, opium poppy, remains critical for the production and supply of morphine, codeine and various semi-synthetic analgesics. Opium poppy was transformed with constitutively expressed cDNA of codeinone reductase (PsCor1.1), the penultimate step in morphine synthesis. Most transgenic lines showed significant increases in capsule alkaloid content in replicated glasshouse and field trials over 4 years. The morphinan alkaloid contents on a dry weight basis were between 15% and 30% greater than those in control high-yielding genotypes and control non-transgenic segregants. Transgenic leaves had approximately 10-fold greater levels of Cor transcript compared with non-transgenic controls. Two cycles of crossing of the best transgenic line into an elite high-morphine genotype resulted in significant increases in morphine and total alkaloids relative to the elite recurrent parent. No significant changes in alkaloid profiles or quantities were observed in leaf, roots, pollen and seed.