Nodulation and effective nitrogen fixation of Macroptilium atropurpureum (siratro) by Burkholderia tuberum, a nodulating and plant growth promoting beta-proteobacterium, are influenced by environmental factors
Nodulation and effective nitrogen fixation of Macroptilium atropurpureum (siratro) by Burkholderia tuberum, a nodulating and plant growth promoting beta-proteobacterium, are influenced by environmental factors
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DOI：
10.1007/s11104-013-1590-7
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发表时间：
2013-08-01
期刊：
影响因子：
4.9
通讯作者：
Hirsch, Ann M.
中科院分区：
文献类型：
--
作者：
Angus, Annette A.;Lee, Andrew;Hirsch, Ann M.
Burkholderia tuberum STM 678（T）分离自南非豆科植物，但不使该植物重新结瘤。在找到可靠的宿主之前，对这种和其他有趣的β-根瘤菌的研究无法取得进展。我们调查了B。tuberum STM 678（T）在小种子、易于繁殖的豆科植物（Macroptilium atropurpureum）上诱导Fix（+）结瘤的能力。以往的研究表明，B。tuberum在siratro上诱导出Fix（-）或Fix（+）结瘤，但造成这种差异的原因尚不清楚。在不同环境条件下进行了促进有效siratro结瘤的实验。B。检查了块茎STM 678（T）耐受高温和干燥的能力以及其通过除固氮之外的机制促进植物生长的潜力，例如，磷酸盐溶解和铁载体的产生。在测序的基因组中发现了这些活动的潜在基因。更高的温度和减少的浇水导致了siratro可靠，有效的繁殖。伯克氏菌属溶解磷酸盐并产生铁载体。Siratro是B的一个很好的模式植物。块茎STM 678（T）。我们确定的基因，可能涉及固氮伯克霍尔德菌物种的生存能力恶劣的条件下，溶解磷酸盐，并产生铁载体。
Burkholderia tuberum STM678(T) was isolated from a South African legume, but did not renodulate this plant. Until a reliable host is found, studies on this and other interesting beta-rhizobia cannot advance. We investigated B. tuberum STM678(T)'s ability to induce Fix(+) nodules on a small-seeded, easy-to-propagate legume (Macroptilium atropurpureum). Previous studies demonstrated that B. tuberum elicited either Fix(-) or Fix(+) nodules on siratro, but the reasons for this difference were unexplored.Experiments to promote effective siratro nodule formation under different environmental conditions were performed. B. tuberum STM678(T)'s ability to withstand high temperatures and desiccation was checked as well as its potential for promoting plant growth via mechanisms in addition to nitrogen fixation, e.g., phosphate solubilization and siderophore production. Potential genes for these activities were found in the sequenced genomes.Higher temperatures and reduced watering resulted in reliable, effective nodulation on siratro. Burkholderia spp. solubilize phosphate and produce siderophores. Genes encoding proteins potentially involved in these growth-promoting activities were detected and are described.Siratro is an excellent model plant for B. tuberum STM678(T). We identified genes that might be involved in the ability of diazotrophic Burkholderia species to survive harsh conditions, solubilize phosphate, and produce siderophores.