High non-esterified fatty acids concentrations promote expression and secretion of fibroblast growth factor 21 in calf hepatocytes cultured in vitro
High non-esterified fatty acids concentrations promote expression and secretion of fibroblast growth factor 21 in calf hepatocytes cultured in vitro
复制标题
高非酯化脂肪酸浓度促进体外培养的小牛肝细胞中成纤维细胞生长因子21的表达和分泌
DOI：
10.1111/jpn.12699
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发表时间：
2018
影响因子：
2.7
通讯作者：
B.Y. Zhao
中科院分区：
文献类型：
--
作者：
J.G. Wang;Y.Z. Guo;Y.Z. Kong;S.H. Dai;B.Y. Zhao
能量负平衡被认为是围产期奶牛能量代谢紊乱的病理基础。血清非酯化脂肪酸（NEFA）是能量平衡状态最重要的指标之一。成纤维细胞生长因子 21 (FGF21) 已被确定为参与代谢适应调节的肝因子，例如在能量剥夺期间促进肝脂质氧化和生酮。然而，NEFA 对牛肝细胞中 FGF21 表达和分泌的直接影响尚不完全清楚。本研究的目的是探讨不同NEFA浓度对体外培养的小牛肝细胞中FGF21表达和分泌的影响。将NEFA以终浓度0.6、1.2、1.8和2.4mmol/L添加到培养液中。连续培养24 h后，分别采用实时PCR和Western blot检测肝细胞中FGF21 mRNA和蛋白表达水平。通过酶联免疫吸附测定(ELISA)测定上清液中的FGF21分泌。结果显示，0.6 mmol/L NEFA处理的肝细胞中FGF21的表达和分泌量高于对照组（P<0.05）。 1.2、1.8和2.4 mmol/L NEFA处理的肝细胞中FGF21的表达和分泌相似，并且显着高于对照组（P<0.01）。这些数据表明高浓度的NEFA显着促进牛肝细胞中FGF21的表达和分泌。特别是，这种促进作用呈剂量依赖性，可能参与了围产期奶牛能量代谢紊乱的病理过程。
Negative energy balance is considered as the pathological basis of energy metabolic disorders in periparturient dairy cows. Serum non-esterified fatty acids (NEFA) is one of the most important indicators of energy balance status. Fibroblast growth factor 21 (FGF21) has been identified as a hepatokine involved in regulation of metabolic adaptations, such as promoting hepatic lipid oxidation and ketogenesis, during energy deprivation. However, the direct effects of NEFA on FGF21 expression and secretion in bovine hepatocytes are not entirely clear. The objective of this study was to investigate the effects of different NEFA concentrations on FGF21 expression and secretion in calf hepatocytes cultured in vitro. NEFA was added to the culture solution at final concentrations of 0.6, 1.2, 1.8 and 2.4 mmol/L. After 24 h of continuous culture, FGF21 mRNA and protein expression levels in the hepatocytes were determined by real-time PCR and Western blot, respectively. FGF21 secretion in the supernatant was determined by enzyme-linked immunosorbent assay (ELISA). The results showed that expression and secretion of FGF21 at 0.6 mmol/L NEFA-treated hepatocytes was higher than that of the control group (P<0.05). The FGF21 expression and secretion were similar at 1.2, 1.8 and 2.4 mmol/L NEFA-treated hepatocytes and significantly higher than those observed for controls (P<0.01). These data suggest that high concentrations of NEFA significantly promote FGF21 expression and secretion in bovine hepatocytes. In particular, this promotion occurs in a dose-dependent manner and may be involved in the pathological processes of energy metabolism disorders of dairy cows in the peripartum period.