CCAAT/enhancer-binding proteins alpha and beta in brown adipose tissue: evidence for a tissue-specific pattern of expression during development.
CCAAT/enhancer-binding proteins alpha and beta in brown adipose tissue: evidence for a tissue-specific pattern of expression during development.
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DOI：
10.1042/bj3020695
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发表时间：
1994-09
期刊：
影响因子：
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通讯作者：
C. Manchado;P. Yubero;O. Viñas;R. Iglesias;Francesc Villarroya;T. Mampel;M. Giralt
中科院分区：
文献类型：
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作者：
C. Manchado;P. Yubero;O. Viñas;R. Iglesias;Francesc Villarroya;T. Mampel;M. Giralt
CCAAT/增强子结合蛋白（C/EBP）α mRNA及其蛋白产物C/EBP α和30 kDa C/EBP α在大鼠棕色脂肪组织中表达。结果还表明，C/EBP β mRNA及其蛋白产物C/EBP β和肝抑制蛋白（LIP）在组织中的表达。成人棕色脂肪中C/EBP α和C/EBP β蛋白的丰度与成人肝脏中发现的相似。然而，C/EBP α和C/EBP β的表达在发育过程中在棕色脂肪中受到特异性调节。C/EBP α，30 kDa C/EBP α，C/EBP β和LIP含量在胎儿棕色脂肪中比在任何发育阶段的成人组织或肝脏中高几倍。峰值在胎儿晚期达到，与解偶联蛋白（UCP）基因（终末棕色脂肪细胞分化的分子标志物）转录的开始同时发生。当成年大鼠暴露于寒冷环境时，这是棕色脂肪组织增生和UCP基因表达的生理刺激，观察到C/EBP β表达相对于C/EBP α、30 kDa C/EBP α和LIP的特异性升高。目前的数据表明，C/EBP家族的转录因子有一个重要的作用，在棕色脂肪组织的发展和终端分化。
CCAAT/enhancer-binding protein (C/EBP) alpha mRNA and its protein products C/EBP alpha and 30 kDa C/EBP alpha are expressed in rat brown-adipose tissue. Results also demonstrate the expression of C/EBP beta mRNA and its protein products C/EBP beta and liver inhibitory protein (LIP) in the tissue. The abundance of C/EBP alpha and C/EBP beta proteins in adult brown fat is similar to that found in adult liver. However, the expression of C/EBP alpha and C/EBP beta is specifically regulated in brown fat during development. C/EBP alpha, 30 kDa C/EBP alpha, C/EBP beta and LIP content is several-fold higher in fetal brown fat than in the adult tissue, or liver at any stage of development. Peak values are attained in late fetal life, in concurrence with the onset of transcription of the uncoupling protein (UCP) gene, the molecular marker of terminal brown-adipocyte differentiation. When adult rats are exposed to a cold environment, which is a physiological stimulus of brown-adipose tissue hyperplasia and UCP gene expression, a specific rise in C/EBP beta expression with respect to C/EBP alpha, 30 kDa C/EBP alpha and LIP is observed. Present data suggest that the C/EBP family of transcription factors has an important role in the development and terminal differentiation of brown-adipose tissue.