INTERACTION BETWEEN BACTERIOPHAGE-SF6 AND SHIGELLA-FLEXNERI
INTERACTION BETWEEN BACTERIOPHAGE-SF6 AND SHIGELLA-FLEXNERI
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DOI：
10.1128/jvi.27.1.38-44.1978
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发表时间：
1978-01-01
影响因子：
5.4
通讯作者：
WOHLHIETER, JA
中科院分区：
文献类型：
--
作者：
LINDBERG, AA;WOLLIN, R;WOHLHIETER, JA
色葡萄弗氏噬菌体Sf6具有直径为53 nm的六角对称的等距头部。非收缩尾长16 nm，终止于含有6个棘突的基板。Sf 6是典型的布拉德利形态分类中的C.噬菌体Sf6具有α-通过对与S.具有O-组3、4抗原的福氏菌株。主要终产物是具有以下结构的八糖：Rha III-GlcNAc-Rha I-Rha II-Rha III-GlcNAc-Rha I-Rha II。O-多糖链的鼠李糖III的0-2的乙酰化，由Sf6溶原化引起或在野生型S.弗氏（3B）菌株阻止酶水解。多糖链的O-脱乙酰化再次使其对Sf6内鼠李糖苷酶敏感。
The S. flexneri phage Sf6 has an isometric head with hexagonal symmetry 53 nm in diameter. The noncontractile tail is 16 nm long and terminates with a base plate containing 6 spikes. Sf6 is typical of the C phages in the morphological classification of Bradley. Phage Sf6 possesses .alpha.-1,3-endorhamnosidase activity as demonstrated by methylation and reducing end group sugar analyses of the products obtained on interaction with the O-polysaccharide chain of S. flexneri strains which have the O-group 3,4 antigen. The major end product was an octasaccharide with the following structure: Rha III-GlcNAc-Rha I-Rha II-Rha III-GlcNAc-Rha I-Rha II. Acetylation of 0-2 of rhamnose III of the O-polysaccharide chain, either brought about by Sf6 lysogenization or found in wild-type S. flexneri (3b) strains, prevented enzymatic hydrolysis. O-deacetylation of the polysaccharide chain again made it susceptible to the Sf6 endorhamnosidase.