Pluronic-coated carbon nanotubes do not induce degeneration of cortical neurons in vivo and in vitro
Pluronic-coated carbon nanotubes do not induce degeneration of cortical neurons in vivo and in vitro
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DOI：
10.1016/j.nano.2008.06.008
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发表时间：
2009-03-01
影响因子：
5.4
通讯作者：
Pizzorusso, Tommaso
中科院分区：
文献类型：
--
作者：
Bardi, Giuseppe;Tognini, Paola;Pizzorusso, Tommaso
碳纳米管（CNTs）是一种在药物和基因传递等生物医学领域具有重要应用前景的纳米器件。目前没有治疗的脑部疾病可能是基于CNT的治疗的候选者。关于碳纳米管的毒性及其在大脑中的分散因子知之甚少。在这里，我们表明，多壁碳纳米管（多壁碳纳米管）涂有普朗尼克F127（PF127）表面活性剂可以注射在小鼠大脑皮层，而不会引起周围的注射部位的神经元变性。我们还表明，与先前关于PF127对培养细胞系缺乏毒性的报道相反，低至0.01%的PF127浓度可以在24小时内诱导体外小鼠原代皮层神经元凋亡。然而，多壁碳纳米管的存在可以避免PF127诱导的细胞凋亡。这些结果表明，PF127涂层多壁碳纳米管不诱导皮层神经元凋亡。此外，MWCNT的存在可以降低PF127的毒性。(C)2009 Elsevier Inc. All rights reserved.
Carbon nanotubes (CNTs) are nanodevices with important potential applications in biomedicine such as drug and gene delivery. Brain diseases with no current therapy could be candidates for CNT-based therapies. Little is known about toxicity of CNTs and of their dispersion factors in the brain. Here we show that multiwall CNTs (MWCNTs) coated with Pluronic F127 (PF127) surfactant can be injected in the mouse cerebral cortex without causing degeneration of the neurons surrounding the site of injection. We also show that, contrary to previous reports on lack of PF127 toxicity on cultured cell lines, concentrations of PF127 as low as 0.01% can induce apoptosis of mouse primary cortical neurons in vitro within 24 hours. However, the presence of MWCNTs can avoid PF127-induced apoptosis. These results suggest that PF127-coated MWCNTs do not induce apoptosis of cortical neurons. Moreover, the presence of MWCNTs can reduce PF127 toxicity. (C) 2009 Elsevier Inc. All rights reserved.