Motivational assessment of mice using the touchscreen operant testing system: effects of dopaminergic drugs.
Motivational assessment of mice using the touchscreen operant testing system: effects of dopaminergic drugs.
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DOI：
10.1007/s00213-015-4009-8
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发表时间：
2015-11
影响因子：
3.4
通讯作者：
Saksida LM
中科院分区：
文献类型：
--
作者：
Heath CJ;Bussey TJ;Saksida LM
触摸屏被广泛用于检测啮齿动物的认知能力。目前的范例要求动物在适当的触摸屏位置查看刺激和鼻子戳。在做出反应后，几乎没有屏幕互动，而且由于红外触摸屏消除了身体接触的需要，体感反馈最小。因此，目前还不清楚触摸屏是否可以支持范式所需的有力的，重复的反应，如评估动机的渐进比（PR）和评估决策的努力相关选择（ERC）。本研究旨在调整和验证啮齿动物触摸屏的PR和ERC。训练雄性C57 B1/6小鼠，直至对PR的反应稳定。通过腹膜内途径给予安非他明、舒必利和雷氯必利以改善性能。将小鼠转移至ERC，并调整范式参数以证明行为改变。ERC奖励偏好通过笼舍选择分析进行评估。公关表现在七个会议内稳定下来。安非他明（1毫克/公斤）增加和雷氯必利（0.3毫克/公斤）降低性能分别为63%和28%，20分钟的注射测试间隔。舒必利（50 mg/kg）在40分钟注射-试验间隔后使性能降低19%。ERC操作性要求的增加从操作性反应依赖的首选奖励转向免费提供的替代品。在触摸屏PR和ERC中可持续进行有力的重复响应，任务验证反映了非触摸屏版本。因此，动机和奖励相关的决策可以直接用触摸屏测量，并且可以在同一设备中的认知测试之前进行评估，以避免被动机因素混淆。
Touchscreens are widely used to examine rodent cognition. Current paradigms require animals to view stimuli and nose poke at an appropriate touchscreen location. After responding, there is little screen interaction and, as infra-red touchscreens eliminate the need for physical contact, minimal somatosensory feedback. It is therefore unclear if touchscreens can support the vigorous, repetitive responding required in paradigms like progressive ratio (PR) for assessing motivation and effort-related choice (ERC) for assessing decision-making. This study aims to adapt and validate PR and ERC for the rodent touchscreen. Male C57Bl/6 mice were trained until responding on PR stabilised. Amphetamine, sulpiride and raclopride were administered via the intraperitoneal route to modify performance. Mice were transferred to ERC and paradigm parameters adjusted to demonstrate behavioural modification. ERC reward preference was assessed by home cage choice analysis. PR performance stabilised within seven sessions. Amphetamine (1 mg/kg) increased and raclopride (0.3 mg/kg) decreased performance by 63 and 28 %, respectively, with a 20-min injection-test interval. Sulpiride (50 mg/kg) decreased performance by 19 % following a 40-min injection-test interval. Increasing ERC operant requirements shifted responding from the operant response-dependent preferred reward towards the freely available alternative. Vigorous, repetitive responding is sustainable in touchscreen PR and ERC and task validation mirrors non-touchscreen versions. Thus, motivation and reward-related decision-making can be measured directly with touchscreens and can be evaluated prior to cognitive testing in the same apparatus to avoid confounding by motivational factors.