Development of a rapid antibody test for point-of-care diagnosis of animal African trypanosomosis
Development of a rapid antibody test for point-of-care diagnosis of animal African trypanosomosis
复制标题
DOI：
10.1016/j.vetpar.2016.11.017
复制
发表时间：
2017-01-15
影响因子：
2.6
通讯作者：
Baltz, Theo
中科院分区：
文献类型：
--
作者：
Boulange, Alain;Pillay, Davita;Baltz, Theo
刚果锥虫（Trypanosoma congolense）和T.间日疟原虫是动物非洲锥虫病（AAT）的主要病原体，这种疾病阻碍了整个撒哈拉以南非洲和南美洲部分地区的畜牧业生产。虽然目前有两种杀锥虫药物，但由于难以在现场诊断这种疾病，治疗水平很低。AAT的主要临床症状，如贫血、体重减轻和不育，与其他几种地方性牲畜疾病相同。目前的诊断方法，基于血液中寄生虫的可视化，或基于检测其DNA或其在宿主中引发的抗体，不适合在现场直接使用，因为它们需要专门的设备和人员。因此，我们开发了一种基于重组TcoCB和TvGM 6抗原的快速诊断试验（15分钟），用于检测T。congolense和T.间日疟，分别旨在为农民和兽医提供在现场进行快速诊断的手段。使用实验感染牛的血清和新鲜血液（如可能）评价试验的特异性和灵敏度。包括两种抗原的原型显示出95.9的特异性（95% C.I.，90.4%-100%）和92.0%（95% C.I.，85.9%~ 98.1%。刚果和98.2%（95% C.I.，94.7%~ 100%。间日疟原虫这种快速检测的高水平灵敏度和特异性，直接使用全血的可能性以及解释结果的容易性，都有助于使这种检测成为有助于在该领域控制AAT的有价值的候选者。然而，为了将该检测与ELISA（目前锥虫病的金标准血清学检测）进行比较，需要使用更具代表性的大量新鲜参考样本进行进一步检测。(C)2016爱思唯尔B. V.保留所有权利。
Trypanosoma congolense and T. vivax are the main causative agents of animal African trypanosomosis (AAT), a disease which hinders livestock production throughout sub-Saharan Africa and in some parts of South America. Although two trypanocidal drugs are currently available, the level of treatment is low due to the difficulty in diagnosing the disease in the field. The major clinical signs of AAT such as anaemia, weight loss, -and infertility, are common to several other endemic livestock diseases. Current diagnostic methods, based on the visualization of the parasite in the blood, or on the detection of its DNA or the antibodies it triggers in the host, are not suitable for direct use in the field as they require specialized equipment and personnel. Thus, we developed a quick-format diagnostic test (15 min) based on the recombinant TcoCB and TvGM6 antigens for detection of T. congolense and T. vivax, respectively, aimed at providing farmers and veterinarians in the field with the means to conduct a quick diagnosis. The specificity and sensitivity of the test were evaluated using sera from experimentally infected cattle, and fresh blood when possible. The prototype, which includes both antigens, shows a specificity of 95.9 (95% C.I., 90.4%-100%) and a sensitivity of 92.0% (95% C.I., 85.9%-98.1%) for T. congolense and 98.2% (95% C.I., 94.7%-100%) for T. vivax. The high levels of sensitivity and specificity of this rapid test, the possibility of using directly whole blood, and the ease of interpreting the result, all contribute to make of this test a valuable candidate to contribute to the control of AAT in the field. However, further tests with more representative, numerous and fresh reference samples are necessary in order to compare this test with the ELISA, the current gold standard serological test for trypanosomosis. (C) 2016 Elsevier B.V. All rights reserved.