Binding event measurement using a chip calorimeter coupled to magnetic beads
Binding event measurement using a chip calorimeter coupled to magnetic beads
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DOI：
10.1016/j.snb.2009.12.012
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发表时间：
2010-03-04
影响因子：
8.4
通讯作者：
Lerchner, J.
中科院分区：
文献类型：
--
作者：
Ahmad, L. M.;Towe, B.;Lerchner, J.
芯片量热法是一种有前途的无标记生物分子检测方法。使用静止液滴的小型量热装置需要纳升范围内的样品体积，但在体积特定信号分辨率、基线稳定性和蒸发方面表现出一些限制。在本文中，我们描述了基于硅芯片的流通量热计在生物素和适体检测中的应用。为了实现高灵敏度，使用了与磁珠偶联的受体。链霉亲和素用作检测生物素的受体。首次成功证明了 DNA 杂交的芯片量热反应。对于这些实验，将生物素化的 DNA 寡聚物互补物偶联至磁珠的链霉亲和素表面。 (C) 2009 Elsevier B.V. 保留所有权利。
Chip calorimetry is a promising method for the label-free detection of biomolecules. Miniaturized calorimetric devices using stagnant droplets require sample volumes in the nano-liter range, but exhibit some limitations regarding volume specific signal resolution, baseline stability, and evaporation. In this paper, we describe the application of a silicon chip based flow-through calorimeter for the detection of biotin and aptamers. To achieve high sensitivity, receptors coupled to magnetic beads were used. Streptavidin served as a receptor for the detection of biotin. For the first time, the chip-calorimetric response to DNA hybridization has been successfully demonstrated. For these experiments, the biotinylated complement of the DNA oligomer was coupled to the streptavidin surface of magnetic beads. (C) 2009 Elsevier B.V. All rights reserved.