Effects of a blend of Saccharomyces cerevisiae-based direct-fed microbial and fermentation products on plasma carbonyl-metabolome and fecal bacterial community of beef steers
Effects of a blend of Saccharomyces cerevisiae-based direct-fed microbial and fermentation products on plasma carbonyl-metabolome and fecal bacterial community of beef steers
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DOI：
10.1186/s40104-019-0419-5
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发表时间：
2020-02-17
影响因子：
7
通讯作者：
Ogunade, Ibukun M.
中科院分区：
文献类型：
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作者：
Adeyemi, James A.;Peters, Sunday O.;Ogunade, Ibukun M.
背景以前的研究已经使用基于酶的测定法评估了直接饲喂微生物（DFM）的动物的代谢状态，该测定法耗时且仅限于少数代谢物。此外，很少强调研究DFM对后肠微生物群的影响。我们通过代谢组学方法研究了日粮中添加基于酿酒酵母的DFM和发酵产物的混合物对血浆中含羰基代谢物浓度的影响，并通过16 S rRNA基因测序研究了42天接收期间肉牛粪便细菌群落。将40头刚断奶的阉牛随机分配接受不含添加剂的基础日粮（CON; n = 20）或补充有19 g Commence（TM）的基础日粮（PROB; n = 20），持续42天。Commence（TM）（PMI，阿登丘陵，MN）是6.2 × 10（11）cfu/g S.酿酒酵母，3.5 × 10（10）cfu/g的乳酸肠球菌、枯草芽孢杆菌、屎肠球菌和干酪乳杆菌的混合物，以及这些上述微生物的发酵产物和黑曲霉和尼日尔曲霉的发酵产物。在第0天和第40天，从每个处理组的10头阉牛中随机采集直肠粪便样本。第42天采血制备血浆。结果共检出812种代谢产物。多达305种代谢产物[倍数变化（FC）>= 1.5，FDR
Background Previous studies have evaluated the metabolic status of animals fed direct-fed microbial (DFM) using enzyme-based assays which are time-consuming and limited to a few metabolites. In addition, little emphasis has been placed on investigating the effects of DFM on hindgut microbiota. We examined the effects of dietary supplementation of a blend of Saccharomyces cerevisiae-based DFM and fermentation products on the plasma concentrations of carbonyl-containing metabolites via a metabolomics approach, and fecal bacterial community, via 16S rRNA gene sequencing, of beef steers during a 42-day receiving period. Forty newly weaned steers were randomly assigned to receive a basal diet with no additive (CON; n = 20) or a basal diet supplemented with 19 g of Commence (TM) (PROB; n = 20) for a 42-day period. Commence (TM) (PMI, Arden Hills, MN) is a blend of 6.2 x 10(11) cfu/g of S. cerevisiae, 3.5 x 10(10) cfu/g of a mixture of Enterococcus lactis, Bacillus subtilis, Enterococcus faecium, and Lactobacillus casei, and the fermentation products of these aforementioned microorganisms and those of Aspergillus oryzae and Aspergillus niger. On d 0 and 40, rectal fecal samples were collected randomly from 10 steers from each treatment group. On d 42, blood was collected for plasma preparation. Results A total number of 812 plasma metabolites were detected. Up to 305 metabolites [fold change (FC) >= 1.5, FDR