Fructose 2,6-bisphosphate. A new activator of phosphofructokinase.
Fructose 2,6-bisphosphate. A new activator of phosphofructokinase.
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DOI：
10.1016/s0021-9258(19)69584-0
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发表时间：
1981-04
期刊：
影响因子：
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通讯作者：
S. Pilkis;M. El‐Maghrabi;J. Pilkis;T. Claus;D. Cumming
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文献类型：
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作者：
S. Pilkis;M. El‐Maghrabi;J. Pilkis;T. Claus;D. Cumming
用DEAE-Sephadex柱层析法从大鼠肝细胞提取物中部分纯化了一种新的磷酸果糖激酶激活剂。在糖二磷酸区域洗脱的活化剂对酸处理敏感，但在碱中耐热。温和的酸水解导致糖单磷酸的出现，其通过气相色谱/质谱被鉴定为果糖6-磷酸。这些观察结果表明，激活剂是果糖2，6-二磷酸。该化合物是通过先将1，6-二磷酸果糖与二环己基碳二亚胺反应，然后用碱处理环状中间体而合成的。合成化合物的结构通过13 C NMR光谱确定为果糖2，6-二磷酸。果糖2，6-二磷酸具有与从肝细胞提取物中纯化的激活剂相同的性质。它在0.1 μ M范围内激活大鼠肝脏和兔骨骼肌酶，比1，6-二磷酸果糖更有效几个数量级。果糖2，6-二磷酸不是醛缩酶或果糖1，6-二磷酸酶的底物。这种新的激活剂可能是磷酸果糖激酶在体内的重要生理因子。
A new activator of rat liver phosphofructokinase was partially purified from rat hepatocyte extracts by DEAE-Sephadex chromatography. The activator, which eluted in the sugar diphosphate region, was sensitive to acid treatment but resistant to heating in alkali. Mild acid hydrolysis resulted in the appearance of a sugar monophosphate which was identified as fructose 6-phosphate by gas chromatography/mass spectroscopy. These observations suggest that the activator is fructose 2,6-bisphosphate. This compound was synthesized by first reacting fructose 1,6-bisphosphate with dicyclohexylcarbodiimide and then treating the cyclic intermediate with alkali. The structure of the synthetic compound was definitively identified as fructose 2,6-bisphosphate by 13C NMR spectroscopy. Fructose 2,6-bisphosphate had properties identical with those of the activator purified from hepatocyte extracts. It activated both the rat liver and rabbit skeletal muscle enzyme in the 0.1 microM range and was several orders of magnitude more effective than fructose 1,6-bisphosphate. Fructose 2,6-bisphosphate was not a substrate for aldolase or fructose 1,6-bisphosphatase. It is likely that this new activator is an important physiologic factor of phosphofructokinase in vivo.